Fluorescence nanoscopy has reached levels of resolution compatible with structural biology measurements. The groups of Stefan Hell and Ralf Jungmann have recently demonstrated position measurements in biological samples with a precision below 1 nm. In contrast to traditional methods like X-ray crystalography or cryo-EM, these measurements can be performed under conditions compatible with life, namely at room temperature and in liquid environment.
In our latest publication, we add a new dimension to these type of measurements: orientation. Santiago Sosa and coworkers present a way of determining the dynamic orientation of a small molecule inside a protein cage, just a few nanometers in size!
These orientational measurements at room temperature and liquid environment enable a new, more realistic, dialogue between experiments and MD simulations.
Santiago Sosa, Alan M. Szalai, Lucía F. Lopez, Juan Manuel Prieto, Cecilia Zaza, Aleksandra K. Adamczyk, Hernan R. Bonomi, Marcelo Marti, Guillermo P. Acuna, Fernando A. Goldbaum, Fernando D. Stefani
