CONICET publicó una muy linda nota sobre el trabajo interdisciplinario que venimos llevando adelante con los grupos de neurobiología de los Dres. Alfredo Cáceres y Damián Refojo, destacando los últimos logros publicados en Nature Structural and Molecular Biology, Nature Communications y Nano Letters. En el artículo titulado "Viendo el interior de las neuronas", se describe [...]
This year, the traditional conference Focus on Microscopy - FOM2021 - was held as an online edition from Sunday March 28 to Wednesday March 31, 2021. Our group participated with three contributions: 1) Luciano Masullo presented pulsed interleaved MINFLUX Luciano A. Masullo, Florian Steiner, Jonas Zähringer, Lucía F. Lopez, Johann Bohlen, Lars Richter, Fiona Cole, [...]
El Prof. Fernando Stefani fue incorporado como Miembro Titular a la Asociación Argentina para el Progreso de las Ciencias. La Asociación Argentina para el Progreso de las Ciencias (AAPC) es una organización no gubernamental sin fines de lucro, dedicada a fomentar el avance y la difusión de la ciencia y la tecnología. Fundada en 1933 [...]
DNA-origami technology is wonderful to fabricate all kinds of nanoscopic objects by self-assembly. We, in particular, use them to fabricate nanophotonic devices. That involves placing photon emitters alongside nanoparticles of different materials on well-defined nanometric geometries. DNA-origami is unique in the sense they provide great positional and stoichiometric control; we can decide where to place [...]
Förster resonance energy transfer (FRET) from a fluorophore in the excited state (donor, D) to a fluorophore in the ground state (acceptor, A) provides a signal sensitive to D−A separation distances in the 0−10 nm range, and to various environmental parameters. A vast number of FRET imaging protocols and sensors have been developed and applied [...]
Single-molecule localization microscopy methods, such as STORM and DNA-PAINT, belong to the most widely used techniques of fluorescence nanoscopy because they provide very good spatial resolution with a simple experimental set-up. STORM reaches lateral resolutions < 20 nm, and DNA-PAINT < 10 nm. However, achieving the same level of resolution in the axial direction of [...]
Since its presentation in early 2017, MINFLUX has generated a lot of interest in the bioimaging community because it enables the localization of single fluorescent molecules with the highest precision, namely 1 nm or less, which is the size of the photon emitting conjugated systems in molecules. However, since then, no other lab in the [...]
Lars Richter came to our group in October 2019 with the support of a DAAD fellowship. For his Master's thesis, he worked closely with Luciano Masullo on our pulsed-interleaved MINFLUX, and was a great member of our research team. Now, Lars has moved to Munich to start a Ph.D. program under the supervision of Prof. [...]
Plasmonic nanoparticles (NPs) are particularly efficient converters of light into heat, making them useful as remote-controlled nanometric sources of heat in numerous applications. However, measuring the temperature of a NP under illumination is not a simple task. In our recent paper in ACS Nano, we present a nanothermometry method based on anti-Stokes photoluminescence emission. Acquiring [...]
SMLM is fantastic to obtain super-resolved fluorescence images. However, it is often the case that the density of localizations is too low to obtain a clear image. Instead, sparse distributions of single-molecule positions are obtained, where any underlying molecular organization is invisible to the eye. The reason for this could be an inefficient labeling or [...]