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# Applied nanoPhysics Group

CIBION

## Sitemaps

- [XML Sitemap](https://stefani-lab.ar/sitemap.xml): Contains all public & indexable URLs for this website.

## Posts

- [Prof. Fernando D. Stefani Joins the Editorial Board of Light: Science & Applications](https://stefani-lab.ar/2025/12/01/prof-fernando-d-stefani-joins-the-editorial-board-of-light-science-applications/) - We are pleased to announce that Prof. Fernando D. Stefani has been appointed as a member of the Editorial Board of Light: Science & Applications (LSA), one of the world’s leading journals in optics and photonics. Published by Nature Portfolio, LSA is a peer‑reviewed, open‑access journal covering all aspects of optics, photonics, and light‑based technologies.
- [FOMISUR 2025: Training the Next Generation in Super‑Resolution Microscopy](https://stefani-lab.ar/2025/11/10/fomisur-2025-training-the-next-generation-in-super-resolution-microscopy/) - From November 4th to 7th, as one of our main activities of the Federal Network of Super-resolution Microscopy, we organized the second edition of FOMISUR (Formación en Microscopía de Super‑Resolución), a hands‑on training workshop dedicated to the principles and applications of super‑resolution fluorescence microscopy. The event brought together students, researchers, and technicians from across Argentina
- [Open-source sub-nanometer stabilization system for super-resolution microscopes](https://stefani-lab.ar/2025/11/21/open-source-sub-nanometer-stabilization-system-for-super-resolution-microscopes/) - All optical microscopes, regardless of how well they are constructed, exhibit some degree of mechanical drift. This drift corresponds to an uncontrolled relative motion between the sample and the objective lens, which can distort images and reduce spatial resolution. A basic research‑grade microscope typically shows a drift on the order of 10–50 nm/min, whereas a
- [Red Federal de Micoscopía de Super-Resolución :  comienza la construcción y el entrenamiento para los dos primeros microscopios](https://stefani-lab.ar/2025/09/05/red-federal-de-micoscopia-de-super-resolucion-comienza-la-construccion-y-el-entrenamiento-para-los-dos-primeros-microscopios/) - Rodrigo Vena y Juan Ignacio Etchart, ambos profesionales de la Carrera de Personal de Apoyo del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) vinieron a CIBION en junio para comenzar a entrenarse en microscopía por ubicación de moléculas individuales y construir sendos microscopios para luego instalar en sus institutos de origen: Rodrigo Vena en
- [Light in South America Conference - Rio de Janeiro](https://stefani-lab.ar/2025/10/18/light-in-south-america-conference-rio-de-janeiro/) - The Light in South America (LiSA) Conference 2025 is a landmark international event co-organized by the Alliance of International Science Organizations (ANSO) and the Brazilian Academy of Sciences, under the framework of the Belt and Road Initiative (BRI) and with the support of journals Light: Science & Applications and eLight. LiSA 2025 aims to unite
- [Celebrating achievements](https://stefani-lab.ar/2023/05/19/celebrating-achievements/) - Today we gathered after work to celebrate publications, grants and awards received by group members. Many thanks to Dr. Piotr Zdankowski for the delicious polish spirits!!
- [Welcome Dr. Piotr Zdankowski](https://stefani-lab.ar/2022/04/01/welcome-dr-piotr-zwandkowski/) - Starting in March 2022, Dr. Piotr Zdankowski has joined our group for a postdoctoral stay with a Research Fellowship of the National Agency for Scientific Exchange of Poland. The Research Fellowship programme aims to implement the Projects together with eminent foreign scientists and scientific teams as well as establish long-term relationship with foreign scientific teams.
- [New group members: Matías Laborero and Lautaro Mancini](https://stefani-lab.ar/2025/03/10/new-group-members-matias-laborero-and-lautaro-mancini/) - Matías Laborero (right) and Lautaro Mancini (left) have joined our group as undergraduate students of the Physics Department of Faculty of Exact and Natural Sciences of the University of Buenos Aires, to make a lab internship. They will implement and train a neural network to clasify astigmatic single moecule signals for 3D single-molecule localization microscopy.
- [PNAS: super-resolution imaging of sperm capacitation](https://stefani-lab.ar/2025/06/10/pnas-super-resolution-imaging-of-sperm-capacitation/) - The protein kinase A (PKA) is an evolutionarily conserved regulator of diverse cellular processes, so advances in understanding its function are impactful. In mammalian sperm, PKA modulates motility and plays a critical role in capacitation, a postejaculation maturation step that enables sperm to fertilize an oocyte. This process depends on PKA regulatory subunits keeping PKA
- [Collaboration with Uni Fribourg - Nature Communications: Single fluorescente molecules on a smartphone](https://stefani-lab.ar/2025/10/10/collaboration-with-uni-fribourg-nature-communications-single-fluorescente-molecules-on-a-smartphone/) - Detecting single molecules is the ultimate detection limit. Among other applications it enables measurements beyond ensemble averaging, ultraprecise analytics and super-resolution imging. Typically, detecting single fluorescent molecules has required costly, research-grade equipment. In our latest publication we present an inexpensive setup that leverages current smartphone technologies to detect single molecules. This project was led by
- [PicoQuant 30th International Workshop on “Single Molecule Spectroscopy and Super-resolution Microscopy”](https://stefani-lab.ar/2025/09/29/picoquant-meeting/) - Since 1995, the company PicoQuant GmbH from Berlin organizes the annual Single Molecule Spectroscopy (SMS) Workshop, which brings together the top researchers in the field, interested scientists from other fields and potential users from life and materials science or industry. This year, for the 30th edition, more than 250 researchers from across the globe gathered
- [Dr. Alan Szalai visiting Prof. Acuna in Fribourg](https://stefani-lab.ar/2025/05/25/dr-alan-szalai-visiting-prof-acuna-in-fribourg/) - Dr. Alan Szalai traveled to Switzerland for a research stay at the group of our collaborator Prof. Guillermo Acuna at the University of Fribourg. Alan will work on finalizing experiments on DNA-PAINT based nanothermometry together with our former member Dr. Mariano Barella, and performing multi-lab SIMPLER measurements.
- [Day of the Department of Physics at Buenos Aires´ Planetarium](https://stefani-lab.ar/2025/08/15/day-of-the-department-of-physics-at-buenos-aires-planetarium/) - On August 14, we participated in the Day of the Department of Physics, a tradition for the past 13 years where the entire Physics Department of the University of Buenos Aires (UBA) gathers in a venue outside the University. This is a wonderful opportunity to continue building the community of the Department and learn about
- [New group member - wellcome Lourdes Simó](https://stefani-lab.ar/2025/08/05/new-group-member-wellcome-lourdes-simo/) - Lourdes Simó has joined our team as undergraduate student of the Physics Department of Faculty of Exact and Natural Sciences of the University of Buenos Aires. She will work on establishing a robust protocol for SIMPLER 3D nanoscopy. Wellcome Lourdes and good luck with your project!
- [Visit to the Vicidomini Lab at the Istituto Italiano de Tecnologia (IIT) Genoa](https://stefani-lab.ar/2025/06/13/visit-to-the-vicidomini-lab-at-the-istituto-italiano-de-tecnologia-iit-genoa/) - On the way to the 12th Weber Symposium, Florencia Choque, Gonzalo Escalante and Dr. Lucía Lopez visited the group of Prof. Giuseppe Vicidomini at the Istituto Italiano de Tecnologia (IIT) Thank you Giuseppe for receving us and showing us the wonderful work you and your team are carrying out. A lot common interests!
- [12th Weber Symposium - Genoa, Italy](https://stefani-lab.ar/2025/06/22/12th-weber-symposium-genoa-italy/) - The 12th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine was held in Genoa, Italy from June 15-20, 2025. The “Weber Symposium” honors the fundamental and far-reaching contributions of Professor Gregorio Weber. It provides an overview of modern fluorescence methodologies and applications in the biological and medical sciences. Weber symposia were held
- [Taller de Óptica y Fotónica (TOPFOT) - Encuentro de Estudiantes de Óptica y Fotónica (EEOF)](https://stefani-lab.ar/2025/05/15/4973/) - We participated in the Taller de Óptica y Fotónica (TOPFOT), an annual workshop organized by Argentina’s Territorial Optics Committee (CTO), serves as a crucial forum for discussing the link between Optics and Photonics and other fields of knowledge, featuring national and international speakers who present open problems. Held concurrently with the TOPFOT is the Encuentro
- [Second round of hands-on training for FOMISUR participants](https://stefani-lab.ar/2025/05/13/second-round-of-hands-on-training-for-fomisur-participants/) - Last December, we organized the first edition of our training workshop on super-resolution microscopy (FOMISUR). We were pleased to receive many more applicants than we could accommodate for the lab practices. As a result, we assigned the available slots by prioritizing participants from more distant regions and scheduled a separate practice date for those near
- [Group asado and farewell to Giovanni](https://stefani-lab.ar/2025/04/08/group-asado-and-farewell-to-giovanni/) - It was a great plasure to have Giovanni Ferrari working with us for the last 4 months. We have advanced our projects and had a lot of fun in the way. Now it is time to go back to Munich, but there is still a lot to do together. Safe travels and see you soon!
- [Wellcome Giovanni Ferrari - visiting graduate student from LMU](https://stefani-lab.ar/2024/12/05/wellcome-giovanni-ferrari-visiting-graduate-student-from-lmu/) - We wellcome Giovanni Ferrari, Ph.D. candidate in the group of Prof. Philip Tinnefeld at the LMU Munich, who is visiting for 4 months with a DAAD fellowship. Giovanni will work on applying p-MINFLUX for single-particle tracking and optimizing a protocolo for widespread use of SIMPLER. We look forward to working with you Giovanni!
- [Guidelines for 3D MINFLUX excitation pattern design](https://stefani-lab.ar/2025/03/10/guidelines-for-minflux-excitation-pattern-design/) - MINFLUX nanoscopy pushes the boundaries of optical resolution by localizing single fluorophores with minimal excitation, providing valuable new insight about the structures and functions of biological systems at the nanoscale. MINFLUX is based on registering the number of photons emitted by the target fluorophore when it is excited with a minimum of light sequentially displaced
- [New group members: Florian Haberkorn Waigel and Facundo Rouquaud](https://stefani-lab.ar/2025/04/01/new-group-members-florian-haberkorn-waigel-and-facundo-rouquaud/) - Florian (right on the picture) has joined the group to pursue a Ph.D. working on single-particle tracking inside human neurons using p-MINFLUX. He has obtained a CONICET fellowship under the direction of Prof. Stefani and co-direction of Dr. Tomás Falzone of IBIOBA. Facundo Rouquaud (left on the picture) has joined as undergraduate student of the
- [Towards fluorescence-based structural biology - Sosa et al. Small Methods](https://stefani-lab.ar/2025/02/02/towards-fluorescence-based-structural-biology-sosa-et-al-small-methods/) - Fluorescence nanoscopy has reached levels of resolution compatible with structural biology measurements. The groups of Stefan Hell and Ralf Jungmann have recently demonstrated position measurements in biological samples with a precision below 1 nm. In contrast to traditional methods like X-ray crystalography or cryo-EM, these measurements can be performed under conditions compatible with life, namely
- [We start the construction of new microscopes for the Federal Network of Super-Resolution Microscopy](https://stefani-lab.ar/2025/02/04/we-start-the-construction-of-new-microscopes-for-the-federal-network-of-super-resolution-microscopy/) - After some delays, we received the funds for the second year of the Federal Network of Super-resolution Microscopy (Red Federal de Microscopía de Super-resolución), and today the first shipment of components arrived. Now we can start building new microscopes to our partners of the network. The goal of the network is to use our open-hardware,
- [Congratulations Dra. Martinez!](https://stefani-lab.ar/2024/11/22/congratulations-dra-martinez/) - Today, Luciana Martinez successfully defended her Ph.D. thesis on optical manipulation, nanothermometry and controlled photo-induced growth of metallic nanoparticles. Next, Luciana will move to University College London to join the group of Sabrina Simoncelli, together with Cecilia Zaza. Congratulations Lu! And good luck in London!
- [Congratulations Dr. Sosa!](https://stefani-lab.ar/2024/11/30/congratulations-dr-sosa/) - Santiago Sosa succesfully defended his Ph.D. thesis. Santi worked under the co-direction of Prof. Fernando Stefani and Prof. Fernando Goldbaum (Leloir Institute) on the engineering of the protein BLS for the fabrications of nanophotonic devices and for developing structural biology measurements based on fluorescence nanoscopy. Congratulations Santi!
- [Night of the Museums 2024](https://stefani-lab.ar/2024/11/11/night-of-the-museums-2024/) - This past Saturday was the Night of the Museums! An annual event organized by the City of Buenos Aires, the Night of the Museums invites everyone to explore the city’s cultural treasures, with museums open from 7:00 PM to midnight, featuring special exhibitions and activities. This year, CIBION participated at the Science Cultural Center (Centro Cultural de la
- [Prof. Stefani is incorporated to the National Academy of Exact, Physical and Natural Sciences](https://stefani-lab.ar/2024/12/02/prof-stefani-is-incorporated-to-the-national-academy-of-exact-physical-and-natural-sciences/) - Prof. Fernando Stefani has been appointed as a Titular Member of the prestigious National Academy of Exact, Physical, and Natural Sciences (ANCEFN). This is a significant honor, as only 37 scholars across all disciplines hold this distinguished position. His inclusion highlights his outstanding contributions to research and innovation, further strengthening the Academy's mission to promote the
- [1st Training Workshop on Super-resolution Microscopy (FOMISUR)](https://stefani-lab.ar/2024/12/13/1st-training-workshop-on-super-resolution-microscopy-fomisur/) - Our lab leads the Federal Network for Super-resolution Microscopy (Red Federal de Microscopía de Super-Resolución) whose main objective is to disseminate super-resolution methods throughout Argentina, comprising hardware, software and “brainware”, i.e. teaching and training. Within this program, we organized the first training workshop on super-resolution microscopy (Taller de Formación en Microscopía de Super-Resolución – FOMISUR).
- [Latin Bioimaging Meeting in Rio de Janeiro](https://stefani-lab.ar/2024/08/24/latin-bioimaging-meeting-in-rio-de-janeiro/) - The Latin Bioimaging (LABI) Meeting in Rio Janeiro was a nice opportunity to meet and discuss with friends and collaborators. Florencia Choque, Alan Szalai, Luis Marcano, Gonzalo Escalante, Santiago Sosa and Fernando Stefani participated, along with collaborators Lía Pietrasanta, Alfredo Cáceres, Francisco Barrantes, Nicolás Unsain, Mariano Bisbal, Antonella Vila, Cayetana Arnaiz, among others. A highlight
- [SAMIC 2024 - Tucumán](https://stefani-lab.ar/2024/05/31/samic-2024-tucuman/) - The Argentina Association of Microscopy (Asociación Argentina de Microscopía – SAMIC), established as non-profit organization in 1995 has been organizing a periodic meetings every two years, approximately, since 2009. This time, the SAMIC meeting was organized in Tucumán, and counted with the participation of numerous national and international colleagues. We would congratulate and thank Dr.
- [Noche de la Ciencia](https://stefani-lab.ar/2023/11/11/noche-de-la-ciencia/) - Our group was part of CIBION’s participation in the event Noche de la Ciencia (Night of Science) showing exciting optics experiments to the public, particularly to kids. A lot of fun indeed!
- [Visit of former Ph.D. student Dr. Federico Barabas](https://stefani-lab.ar/2024/05/07/visit-of-former-ph-d-student-dr-federico-barabas/) - Thank you Fede for dropping by!
- [Optical printing of colloidal nanoparticles moves to UNSAM](https://stefani-lab.ar/2024/03/06/optical-printing-of-colloidal-nanoparticles-moves-to-unsam/) - Dr. Julián Gargiulo and Dr. Ianina moved to the Institute of Nanosystems at the National University of San Martín (Buenos Aires, Argentina) to start a new group on nanophotonics. To support them and help them start with maximum momentum, we have lend them a full setup of topical manipulation and spectroscopy of nanoparticles. The early
- [Prof. Stefani receives the Konex award - 100 renowned argentine scientists of the last decade](https://stefani-lab.ar/2023/09/12/prof-stefani-receives-the-konex-award-100-renowned-argentine-scientists-of-the-last-decade/) - The director of CIBION, Fernando Stefani, received a Konex Merit Award for being one of the 100 most outstanding scientific personalities of the last decade (2013-2022) in Argentina. This award has been given by the Konex Foundation since 1980, on differente categories. This year, the award was for those who excelled in Science and Technology.
- [Prof. Stefan Maier receives the Luis Federico Leloir Award](https://stefani-lab.ar/2023/10/19/prof-stefan-maier-receives-the-luis-federico-leloir-award/) - Congratulations to Prof. Stefan Maier, on being awarded the prestigious Leloir Prize 2023 in the the natural sciences category from Argentina’s Ministry of Science, Technology and Innovation. Prof. Maier is the head of the School of Physics and Astronomy at Monash University, Australia, while he also holds the Lee-Lucas Chair in Experimental Physics and leads head
- [Alan is back!](https://stefani-lab.ar/2023/07/01/alan-is-back/) - Dr. Alan Szalai returns to our group after a succesful stay in the group of our collaborator Prof. Philip Tinnefeld Alan joins now as assistant researcher to start his career at CONICET. We are so happy to have Alan back with us. Undoubtedly, he will boost our research, generate new research lines, and make rapid
- [Asado de fin de año de CIBION](https://stefani-lab.ar/2023/12/20/asado-de-fin-de-ano-de-cibion/) - Great fun at the yearly BBQ of CIBION 🙂
- [Prof. Vahid Sandoghdar visit us](https://stefani-lab.ar/2024/02/27/prof-vahid-sandoghdar-visit-us/) - Prof. Vahid Sandoghdar, director of the Nano-Optics division of the Max Planck Institute for the Science of Light, visited us and gave a talk about his latest research. Prof. Sandoghdar is one of the most important pioneers of nano-optics. He has made breakthrough experiments of single molecule spectrocopy and single nanoparticle detection and tracking. His
- [Group retreat in Buenos Aires province](https://stefani-lab.ar/2024/01/29/group-retreat-in-buenos-aires-province/) - We made a one day trip to the countryside to have some asado and relax. Ana Wendel, Lucía Lopez, Ianina Violi, Gonzalo Escalante, Fernando Stefani, Luciana Martínez, Alan Szalai, Lars Richter (former member of our group visiting us from Germany)
- [Annual Meeting of the Argentina Association of Physics (RAFA 2023) - Premio J. J. Giambiagi a Luciano Masullo](https://stefani-lab.ar/2023/09/23/annual-meeting-of-the-argentina-association-of-physics-rafa-2023-premio-j-j-giambiagi-a-luciano-masullo/) - Several members of the group attended the Annual Meeting of the Argentina Association of Physics in Bahía Blanca to enjoy wonderful scientific discussions and accompany Dr. Luciano Masullo in his presentation on the ocassion of the Juan José Giambiagi Award to the best doctoral thesis in experimental Physics of Argentina defended in the period 2020-2021.
- [Luciano Masullo awarded as Rising Star of Light 2023](https://stefani-lab.ar/2023/11/01/luciano-masullo-awarded-as-rising-star-of-light-2023/) - Fomer member of our group Dr. Luciano Masullo has recevied the Rising Stars of Light award in the “Before Faculty” track. The Rising Stars of Light is as a worldwide campaign initiated by Light: Science & Applications in 2018 to highlight the most shining young scientists in optics-related topics. Since 2021, Rising Stars of Light
- [Annual Meeting of the Biophysical Society 2024 - Invited talk at the Molecular Fluorescence Subgroup](https://stefani-lab.ar/2024/02/15/annual-meeting-of-the-biophysical-society-2024-invited-talk-at-the-molecular-fluorescence-subgroup/) - Prof. Stefani was invited by the Molecular Fluorescence Subgroup of the Biophysical Society to give a lecture on “Fluorescence nanoscopy with sub-10 nm resolution” at the Annual Meeting of the Biophysical Society 2024, held in Philadelphia from the 10th to 14th of February. Many thanks to the orgenizers Dr. Hugo Sanabria and Prof. Claus Seidel.
- [Visit to the Italian Institute of Technology in Genoa hosted by Prof. Giuseppe Vicidmini and with the company of the great Prof. Alberto Diaspro](https://stefani-lab.ar/2023/11/03/visit-to-the-italian-institute-of-technology-in-genoa-hosted-by-prof-giuseppe-vicidmini-and-with-the-company-of-the-great-prof-alberto-diaspro/) - Prof. Stefani visited the very creative group of Prof. Giuseppe Vicidomini at the Istituto Italiano di Tecnologia (IIT) in Genoa to give a lecture and share the latest developments on pulsed-interleaved MINFLUX, RASTMIN and related methodologies. Thank you Giuseppe for the hospitality and great discussions. Also, many thanks to the great Alberto Diaspro for a
- [Night of the Museums 2023](https://stefani-lab.ar/2023/09/24/night-of-the-museums-2023/) - This year CIBION participated in the Nights of the Museums of the City of Buenos Aires together with the Intitute of Biomedicine of Buenos Aires (IBIOBA) and the Center for Simluations and Calculations (CSC). Our group made exciting demonstrations of optics and fluorescence to hundreds of people that visited our stands in the Science Cultural
- [Lecture at the MPI for Multidisciplinary Sciences hosted by Prof. Stefan Hell and with the company of the great Dr. Tom Jovin](https://stefani-lab.ar/2023/04/16/lecture-at-the-mpi-for-multidisciplinary-sciences-hosted-by-prof-stefan-hell/) - Prof. Stefan Hell invited Prof. Stefani to give a lecture at the Max Planck Institute for Multidisciplinary Sciences on the latest advances on super-resolution microscopy with sub-10 nm resolution. This was the first meeting in person after the COVID-19 pandemic with long term collaborators and mentors of Prof Stefani, Prof. Stefan Hell and Prof. Thomas
- [Destacan nuestra investigación en calentamiento fototérmico de nanopartículas](https://stefani-lab.ar/2023/08/25/destacan-nuestra-investigacion-en-calentamiento-fototermico-de-nanoparticulas/) - CONICET publicó una nota de divulgación destacando nuestra investigación sobre el calentamiento fototérmico de nanopartículas bimetálicas de oro y paladio. Este trabajo fue liderado por el Dr. Julián Gargiulo en nuestro grupo, y actualmente mudándose a la Universidad de San Martín, en colaboración con el grupo del Prof. Emiliano Cortés de la Universidad Ludwig-Maximilians de
- [Lecture at University of Göttingen hosted by Prof. Joerg Enderlein](https://stefani-lab.ar/2023/04/14/lecture-at-university-of-gottingen-hosted-by-prof-joerg-enderlein/) - Prof. Stefani was invited by Prof. Joerg Enderlein to a give a coloquium at the University of Göttingen and discuss future collaborations with his group. He also maintained promissing disscussions with Prof. Silvio Rizzoli and group leader Dr. Felipe Opazo. Thank you Joerg for the great time!
- [Visit and course on Advanced Fluorecence Imaging at the University of Fribourg](https://stefani-lab.ar/2023/04/28/visit-and-course-on-advanced-fluorecence-imaging-at-the-university-of-fribourg/) - Prof. Stefani was invited to the University of Fribourg to give lectures and supervise ongoing collaboration projects with Prof. Guillermo Acuna. In addition, Prof. Stefani gave a short course on Advanced Fluorescence Imaging for graduate and undergraduate students of the University of Fribourg and the University of Bern. The course was organized by the head
- [Lecture and visit to Prof. Romain Quidant and Prof. Lukas Novotny at ETH](https://stefani-lab.ar/2023/03/25/lecture-and-visit-to-prof-romain-quidant-and-prof-lukas-novotny-at-eth/) - Prof. Stefani was invited to the ETH Zurich to give a lecture to graduate and undergraduate students, and visit the labs of world experts on nanophotonics Prof. Lukas Novotny and Prof. Romain Quidant. Thank you Romain and Lukas for the great time and discussions, as always.
- [Visit of Prof. Joerg Enderlein](https://stefani-lab.ar/2023/01/18/visit-of-prof-joerg-enderlein/) - We received Prof. Joerg Enderlein from the University of Goettingen at CIBION to show him our labs and discuss current status and future trends of our many common interests, like single-molecule spectroscopy and super-resolution microscopy. Prof. Enderlein is a world expert on single-molecule detection, electromagnetic manipulation of fluorescence states, and super-resolution microscopy schemes. With Prof.
- [Participation in the 11th Weber Symposium](https://stefani-lab.ar/2023/01/12/participation-in-the-11th-weber-symposium/) - Ph.D. candidate Florencia Choque and Prof. Stefani participated in the 11th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine held in Punta del Este, Uruguay, on January 8-13, 2023. The “Weber Symposium” honors the fundamental and far-reaching contributions of Professor Gregorio Weber. It provides an overview of modern fluorescence methodologies and applications in
- [Prof. Stefani visits friends and collaborators in Munich](https://stefani-lab.ar/2022/09/20/visit-to-friends-and-collaborators-in-munich/) - During three weeks in September, Prof. Stefani visited friends and collaborators en the Munich area. He gave lectures and supervised ongoing projects in collaboration. Prof. Stefani visited: Prof. Philip Tinnefeld and his group at the Faculty for Chemistry and Pharmacy of the LMU Munich, where former members Dr. Alan Szalai and Ph. D. candidate Lars
- [Presentations a the annual meeting of the Argentine Society of Biophysics (SAB 2022)](https://stefani-lab.ar/2022/11/18/presentations-a-the-annual-meeting-of-the-argentine-society-of-biophysics-sab-2022/) - This year, the annual meeting of the SAB was held in Rosario. CIBION contributed with the folowing presentations: Gonzalo Escalante – “Super-resolution of membrane proteins in Trypanosoma cruzi” Santiago Sosa – “Determining the orientation of single molecules in a protein cage by fluorescence nanoscopy” Luis Marcano-García – “Determination of association equilibrium constant from single molecule
- [Talk by Luciana Martínez at JOBION 2022](https://stefani-lab.ar/2022/11/25/talk-by-luciana-martinez-at-jobion-2022/) - Ph. D. candidate Luciana Martínez gave a talk at the 4th edition of JOBION held at the Centro Cultural de la Ciencia, in Buenos Aires. Luciana presented her research on the challenges of optical printing metallic nanoparticles of complex shape, particularly gold nanostars. JOBION is a self-organized regular meeting where young researchers working in diverse fields
- [Thank you Pedro!](https://stefani-lab.ar/2022/12/16/thank-you-pedro/) - Friends, students, colleagues and collaborators of Pedro Aramendía got together at the Faculty of Natural and Exact Sciences of the University of Buenos Aires to celebrate his career and say thanks for so many years of great work and company. Pedro was the founding Director of CIBION and was in charge until early 2022. He
- [Our homage to the football world champions :-)](https://stefani-lab.ar/2022/12/30/our-homage-to-the-football-world-champions/) - To honour the effort and great performance of our football national team, Luciana Martínez used our optical printing setup to make a “nano FIFA world cup” made of gold nanoparticles. The FIFA world cup is made of about 6 kilograms of gold 18k, which is about 4,5 kilograms of pure gold. The nano world cup
- [Visit to our collaborator Prof. Guillermo Acuna](https://stefani-lab.ar/2022/06/30/visit-to-our-collaborator-prof-guillermo-acuna/) - During two weeks of June, Prof. Stefani visited our close collaborator Prof. Guillermo Acuna at the University of Fribourg, Switzerland, where several former members of our group are currently working, like Dr. Mariano Barella and Ph.D. candidate Germán Chiarelli. Prof. Stefani gave lectures on the recent advances of our lab, and supervised projects in collaboration
- [Anti-stokes thermometry of single Au/Pd nanoparticles](https://stefani-lab.ar/2023/06/24/anti-stokes-thermometry-of-single-au-pd-nanoparticles/) - Metallic nanoparticles can be used as nanoheaters; they are very efficient converters of light energy into heat. However, accurate measurement of the temperatures reached by a metallic nanoparticle under illumination remains an open challenge, creating difficulties in the interpretation of results across plasmonic applications. Particularly, there is a quest for understanding the role of temperature
- [Super-resolved FRET imaging by confocal single-molecule localization and lifetime](https://stefani-lab.ar/2023/05/25/super-resolved-fret-imaging-by-confocal-single-molecule-localization-and-lifetime/) - Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited
- [Tracking nanoscopic motion with minima of light](https://stefani-lab.ar/2023/06/16/tracking-nanoscopic-motion-with-minima-of-light/) - Many physical, chemical, and biological phenomena are determined by dynamical processes occurring at the nanoscale. Therefore, tracking (bio)molecules and nanoparticles with nanometric precision and high temporal resolution is a truly powerful discovery tool in various fields. When it comes to biology, tracking the motion and conformational changes of proteins lies at the core of deciphering
- [Arrays of gold nanostars by optical printing](https://stefani-lab.ar/2023/03/23/arrays-of-gold-nanostars-by-optical-printing/) - Obtaining arrays of single nanoparticles with three-dimensional complex shapes is of interest for many applications, but remains an open challenge of nanotechnology. Current nanolithographic methods do not allow for the preparation of nanoparticles with complex features like nanostars. In our recent paper in Nano Letters, we present an investigation of the optical printing of gold
- [Konex Award 2023 to Prof. Fernando Stefani](https://stefani-lab.ar/2023/05/02/konex-award-2023-to-prof-fernando-stefani/) - Since 1980, every 10 years, the Konex Foundation (https://www.fundacionkonex.org/) recognizes 100 remarkable personalities or institutions of Argentina for their work over one decade in different areas: Sports, Show Business, Visual Arts, Science and Technology, Literature, Popular Music, Humanities, Comunication, Journalism, Community and Institutions, Enterprises, and Classical Music. This year was the turn of Science
- [Tutorial for the optimization and characterization of toroidal foci for super-resolution fluorescence microscopy](https://stefani-lab.ar/2023/03/30/tutorial-for-the-optimization-and-characterization-of-toroidal-foci-for-super-resolution-fluorescence-microscopy/) - Several super-resolution methods make use of toroidal foci, i.e. light foci with a central minimum, ideally a zero, of intensity. For example, STED microscopy uses toroidal foci to suppress fluorescence emission in the periphery of the excitation focus through stimulated emission. Also, interrogating molecular position using a minimum of intensity is the basis for the
- [New international project funded by the Chan Zuckerberg Initiative to develop Napari plugin](https://stefani-lab.ar/2022/11/12/new-international-project-funded-by-the-chan-zuckerberg-initiative-to-develop-napar-plugin/) - We are excited to be among the international grantees of the Chan Zuckerberg Initiative for the program @napari_imaging This project is an international collaboration with our former member Dr. Luciano Masullo, now at the MPI for Biochemistry, Munich) and Prof. Andrea Bassi from the Politecnico di Milano. We will produce a plugin to simulate the optical
- [CIBION bbq 2022](https://stefani-lab.ar/2022/12/14/cibion-bbq-2022/) - Nothing else to say... like every year we had our yearly get together of the whole institute, and it was great fun :-)
- [Nanometer Resolution Imaging and Tracking of Single Fluorophores by Sequential Structured Illumination](https://stefani-lab.ar/2022/11/19/nanometer-resolution-imaging-and-tracking-of-single-fluorophores-by-sequential-structured-illumination/) - Localization of single fluorescent emitters is central for many physicochemical and biophysical measurements at the nanoscale and beyond ensemble averaging. Examples include single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Among the numerous localization methods available, MINFLUX constituted a breakthrough for achieving a ∼10-fold improvement in resolution over wide-field camera-based approaches, reaching the molecular
- [Best thesis on experimental Physics of the Argentine Physics Association to Dr. Luciano Masullo!](https://stefani-lab.ar/2022/09/29/best-thesis-on-experimental-physics-of-the-argentina-association-of-physics-to-dr-luciano-masullo/) - Luciano Masullo has been awarded with the Juan José Giambiagi award of the Argentine Physics Association (AFA) for the best doctoral thesis on experimental Physics for the period 2020-2021. Luciano joined our group in 2014, for his graduation thesis. At that time he worked closely with Dr. Fede Barabas, learning SMLM and developing the Python
- [Ultracompact and unidirectional optical nano antennas](https://stefani-lab.ar/2022/07/31/ultracompact-and-unidirectional-optical-nano-antennas/) - By nature, photon emission occurs without directionality. In most situations photon emission is dipolar, and multipolar emission may be obtained under very special conditions. Therefore, imposing directionality requires the mediation of specific devices. Directional optical nano antennas have been demonstrated over a decade ago using the Yagi-Uda concept; i.e. a phased array that produces constructive
- [Back to in-person meetings! Day of the Physics Department](https://stefani-lab.ar/2022/08/16/back-to-in-person-meetings-day-of-the-physics-department/) - We are very happy that the traditional annual get together of the Physics Department is back, and that we can discuss science and education face-to-face with our colleagues and friends! The Day of the Physics Department (Día del DF, DDF) is a great meeting where professors, researchers, graduate and undergraduate students discuss the
- [Assembling single molecules with controlled orientation](https://stefani-lab.ar/2022/09/07/4331/) - An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control and well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up nanofabrication methodology that fulfils almost all of these features. It enables the nanometric positioning of molecules and nanoparticles with stoichiometric control, and even
- [Interviews to Luciano Masullo, Lucía Lopez and Fernando Stefani](https://stefani-lab.ar/2022/11/03/interviews-to-luciano-masullo-lucia-lopez-and-fernando-stefani/) - Focal Plane has made a series of interviews to latin american microscopy scientists. Three members of our lab were interviewed to tell about their experiences: An interview with Fernando Stefani - FocalPlane (biologists.com) An interview with Lucía López - FocalPlane (biologists.com) An interview with Luciano Masullo - FocalPlane (biologists.com)
- [Dr. Lucía Lopez speaks at the Latin American Bioimaging meeting 2022](https://stefani-lab.ar/2022/09/13/dr-lucia-lopez-speaks-at-the-latin-american-bioimaging-meeting-2022/) - This year, the meeting of the Latin American Bioimaging network was held in Montevideo. Dr. Lucía Lopez gave an inspiring talk sharing her experience, as a woman imaging scientist in Latin America Dr. Lía Pietrasanta, coordinator of the National System of Microscopy of Argentina was also there, and Prof. Stefani gave a virtual keynote lecture
- [First iCANX talk by a latin american scientist: Prof. Oscar Martinez. Prof. Stefani accompanies as panelist.](https://stefani-lab.ar/2022/07/22/first-icanx-talk-by-a-latin-american-scientist-prof-oscar-martinez-prof-stefani-accompanies-as-panelist/) - The iCANX talks is a wonderful initiative by Prof. Haixia (Alice) Zhang from Pekin University. Every Friday a new talk by top scientists from around the world. You can check them out here https://ican-x.com/talks This friday, it was the turn of Oscar Martinez, former Professor of the Physics Department, currently at the Faculty of Engineering
- [An alternative to MINFLUX that enables nanometre resolution in a confocal microscope](https://stefani-lab.ar/2022/06/01/an-alternative-to-minflux-that-enables-nanometre-resolution-in-a-confocal-microscope/) - Fluorescence microscopy is a major workhorse in life sciences, biophysics, and physical chemistry, as it allows visualization with great specificity and sensitivity. In particular, the detection of single fluorescent molecules has been used to provide information beyond ensemble averages with applications in different fields of research. For example, super-resolution methods based on the localization of
- [Prof. Stefani appointed member of the Editorial Advisory Board of ACS Photonics](https://stefani-lab.ar/2022/03/05/prof-stefani-appointed-member-of-the-editorial-advisory-board-of-acs-photonics/) - Prof. Fernando Stefani has been appointed to the Editorial Advisory Board of ACSPhotonics, becoming the only member from Latin America. ACS Photonics is a reference journal in the field of photonics, and has recently updated the list of topics covered under the new Editor in Chief Prof. Romain Quidant: Nanophotonics, including plasmonics and polaritonics, optical
- [Happy birthday Prof. Jochen Feldmann!](https://stefani-lab.ar/2022/07/17/happy-birthday-prof-jochen-feldmann/) - Prof. Jochen Feldmann has been a great scientist and mentor for many younger researchers. On the occasion of his 60th birthday, several of his mentorees and collaborators have contributed to a special issue of Advances Optical Materials in his honor. Happy birthday Jochen!!! Advanced Optical Materiales - Special Issue Photonics and Optoelectronics of
- [Fine-tuning the light-induced growth of nanoparticles with a luminescence closed-loop control](https://stefani-lab.ar/2022/04/20/fine-tuning-the-light-induced-growth-of-nanoparticles-with-a-closed-loop-control/) - The morphology and composition of metallic nanoparticles (NPs) determine their optical response, so finely controlling them at the single particle level is a key to achieve tailored functionalities. In a recent publication by Luciana Martinez et al. we show that the plasmon-driven growth of Au NPs can be monitored in real time through photoluminescence measurements.
- [New laser thanks to the Alexander von Humboldt Foundation!](https://stefani-lab.ar/2022/08/24/new-laser-thanks-to-the-alexander-von-humboldt-foundation/) - The Alexander von Humboldt Foundation has granted us an Equipment Subsidy to purchase a new supercontinuum laser that will be used for future pulsed-MINFLUX experiments. Ph. D. candidate Florencia Choque already set it up and running. Thanks so much to the Humboldt Foundation for this great support!
- [Prof. Dr. Pedro Aramendía retires and CIBION say thanks](https://stefani-lab.ar/2022/03/31/prof-dr-pedro-aramendia-retires-and-cibion-say-thanks/) - Founding Director of CIBION Prof. Dr. Pedro Aramendía has retired after 10 years leading the activities of the institute. Pedro has been a great researcher, teacher, and mentor of many young researchers. Thank you Pedro for all your work and help during this years! Enjoy your next projects in this new stage of your life.
- [Congratulations to Dr. Cecilia Zaza](https://stefani-lab.ar/2022/04/12/congratulations-to-dr-cecilia-zaza/) - Today, Cecilia "Chechu" Zaza defended her Ph. D. thesis “Impresión óptica de nanopartículas de silicio y métodos para medir temperatura con resolución espacial en la nanoescala”, obtaining the highest grade from the jury Dr. Gustavo Grinblat, Dr. Ana Sol Peinetti, and Dr. Ana María Huergo. Chechu joined the group in 2017 to work on her
- [Multiphoton single-molecule localization with sequential structured illumination](https://stefani-lab.ar/2022/03/26/multiphoton-single-molecule-localization-with-sequential-structured-illumination/) - MINFLUX represented a breakthrough showing that single fluorescent molecules could be localized with 1-2 nm precision with moderate photon counts.Such level of localization efficiency enables single-molecule tracking and super-resolution imaging with true molecular resolution. The key concept was to estimate the molecular position from a sequence of exposures to spatially displaced a light minimum. Expanding
- [Python package for fully vectorial calculations of focused fields](https://stefani-lab.ar/2022/03/03/python-package-for-fully-vectorial-calculations-of-focused-fields/) - Focused optical fields are key to a multitude of applications involving light-matter interactions, such as optical microscopy, single-molecule spectroscopy, optical tweezers, lithography, or quantum coherent control. A detailed vectorial characterization of the focused optical fields that includes a description beyond the paraxial approximation is key to optimize technological performance as well as for the design
- [Review (and new data) on optical printing of colloidal nanoparticles](https://stefani-lab.ar/2022/01/19/review-on-optical-printing-of-colloidal-nanoparticles/) - While colloidal chemistry provides ways to obtain a great variety of nanoparticles with different shapes, sizes, material compositions, and surface functions, their controlled deposition and combination on arbitrary positions of substrates remain a considerable challenge. Over the last ten years, optical printing arose as a versatile method to achieve this purpose for different kinds of
- [A common framework for single-molecule localization with sequential structured illumination (MINFLUX, orbital tracking, MINSTED, etc.)](https://stefani-lab.ar/2022/01/02/a-common-framework-for-single-molecule-localization-with-sequential-structured-illumination-minflux-orbital-tracking-minsted-etc/) - Localization of single fluorescent molecules is key for physicochemical and biophysical measurements, such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Over the last two decades, several methods have been developed in which the position of a single emitter is interrogated with a sequence of spatially modulated patterns of light. Among them, the
- [Review on fluorescence nanoscopy with sub-10 nm resolution](https://stefani-lab.ar/2021/12/02/review-on-fluorescence-nanoscopy-with-sub-10-nm-resolution/) - Fluorescence nanoscopy, also known as super-resolution microscopy, represented a breakthrough for the life sciences as it delivers 20–30 nm resolution using far-field fluorescence microscopes. This resolution limit is not fundamental but imposed by the limited photostability of fluorophores under ambient conditions. This has motivated the development of a second generation of fluorescence nanoscopy methods that
- [Review on super-resolved FRET measurements](https://stefani-lab.ar/2021/11/01/review-on-super-resolved-fret-measurements/) - Super-resolution fluorescence microscopy and Förster Resonance Energy Transfer (FRET) form a well-established family of techniques that has provided unique tools to study the dynamic architecture and functionality of biological systems, as well as to investigate nanomaterials. In the last years, the integration of super-resolution methods with FRET measurements has generated advances in two fronts. On
- [Exciton diffusion and energy transfer in conjugated polymer nanoparticles](https://stefani-lab.ar/2021/10/20/exciton-diffusion-and-energy-transfer-in-conjugated-polymer-nanoparticles/) - Energy transfer in conjugated polymers is a critical process that affects the performance of these materials in diverse applications such as biological–chemical–physical sensing, imaging, photovoltaics, and phototherapy. In this experimental and theoretical work led by Prof. Rodrigo Palacios (University of Río Cuarto, Córdoba, Argentina), we performed an in-depth study of energy transfer in nanoparticles of
- [Farewell Alan and Lucho - best luck in Munich!](https://stefani-lab.ar/2021/09/24/farewell-alan-and-lucho-best-luck-in-munich/) - Dr. Luciano (Lucho) Masullo and Dr. Alan Szalai leave the group after several years of very productive work. Goodbye beer - 24.09.2021 Lucho joined our group in 2014 as an undergraduate student, and stayed with us for almost 7 years! During that time, he worked on STORM, developed pulsed-interleaved MINLFUX and RASTMIN. Next, he
- [Colloquium by Prof. Philip Tinnefeld](https://stefani-lab.ar/2021/09/03/colloquium-by-prof-philip-tinnefeld/) - Today, Prof. Dr. Philip Tinnefeld, one of our closest collaborators, gave a great talk in the colloquium of CIBION, showing some of the wonderful applications of DNA-origami his group develops, ranging from nano-rulers for fluorescence nanoscopy, self-assembled optical antennas and single-molecule biosensing assays. Thank you Philip for sharing your most recent advances and for the
- [Congrats Dr. Luciano Masullo!](https://stefani-lab.ar/2021/08/30/congrats-dr-luciano-masullo/) - Today, Luciano Masullo defended his thesis "Photon-efficient fluorescence nanoscopy by scanning light intensity minima" and obtained the highest grade from the jury (Prof. Dr. Oscar Martínez, Prof. Dr. Valeria Levi, and Prof. Dr. Jorge Torga). Luciano joined our group in 2014, for his graduation thesis. At that time he worked closely with Dr. Fede Barabas,
- [Congratulations Florencia!](https://stefani-lab.ar/2021/05/21/congratulations-florencia/) - Today, Florencia Edorna obtained her degree in Physics from the University of Buenos Aires with the highest grade. Florencia came to our group wishing to work on 3D STED and MINFLUX nanoscopy, incorporating adaptive optics schemes to bring those methods to their maximum performance by correcting aberrations in the optical system and the samples. Unfortunately,
- [Congratulations Fernando Caprile!](https://stefani-lab.ar/2021/05/18/congratulations-fernando-caprile/) - Today, Fernando Caprile obtained his degree in Physics from the University of Buenos Aires with the highest grade. Fernando came to our group wishing to perform single-molecule experiments and to learn the latest advances in fluorescence nanoscopy, in particular about MINFLUX. Unfortunately, the COVID-19 outbreak made it impossible for us to go to the lab.
- [Prof. Stefani es incorporado a la Asociación Argentina para el Progreso de las Ciencias](https://stefani-lab.ar/2021/03/13/prof-stefani-es-incorporado-a-la-asociacion-argentina-para-el-progreso-de-las-ciencias/) - El Prof. Fernando Stefani fue incorporado como Miembro Titular a la Asociación Argentina para el Progreso de las Ciencias. La Asociación Argentina para el Progreso de las Ciencias (AAPC) es una organización no gubernamental sin fines de lucro, dedicada a fomentar el avance y la difusión de la ciencia y la tecnología. Fundada en 1933
- [Divulgación - Viendo el interior de las neuronas](https://stefani-lab.ar/2021/04/13/divulgacion-viendo-el-interior-de-las-neuronas/) - CONICET publicó una muy linda nota sobre el trabajo interdisciplinario que venimos llevando adelante con los grupos de neurobiología de los Dres. Alfredo Cáceres y Damián Refojo, destacando los últimos logros publicados en Nature Structural and Molecular Biology, Nature Communications y Nano Letters. En el artículo titulado "Viendo el interior de las neuronas", se describe
- [Focus on Microscopy 2021](https://stefani-lab.ar/2021/03/31/focus-on-microscopy-2021/) - This year, the traditional conference Focus on Microscopy - FOM2021 - was held as an online edition from Sunday March 28 to Wednesday March 31, 2021. Our group participated with three contributions: 1) Luciano Masullo presented pulsed interleaved MINFLUX Luciano A. Masullo, Florian Steiner, Jonas Zähringer, Lucía F. Lopez, Johann Bohlen, Lars Richter, Fiona Cole,
- [3D TIRF nanoscopy with sub-10 nm resolution](https://stefani-lab.ar/2021/01/23/3d-tirf-nanoscopy-with-sub-10-nm-resolution/) - Single-molecule localization microscopy methods, such as STORM and DNA-PAINT, belong to the most widely used techniques of fluorescence nanoscopy because they provide very good spatial resolution with a simple experimental set-up. STORM reaches lateral resolutions < 20 nm, and DNA-PAINT < 10 nm. However, achieving the same level of resolution in the axial direction of
- [Revealling the NEDDylome](https://stefani-lab.ar/2020/02/28/revealling-the-neddylome/) - We are happy to see our contribution with super-resolution fluorescence imaging to this wonderful work led by our collaborator Dr. Damián Refojo (IBIOBA-CONICET, Max-Partner Institute), and his colleagues from Genentech Dr. Donald S. Kirkpatrick and Dr. Morgan Sheng. Neddylation is the post-translational protein modification most closely related to ubiquitination. Whereas the ubiquitin-like protein NEDD8 is
- [Workshop on the Advances of Super-resolution Optical](https://stefani-lab.ar/2013/11/24/workshop-advances-super-resolution-optical/) - From 18th to 22nd of November, 2013, we held at CIBION The Workshop on the Advances of Super-resolution Optical Microscopy. Methodologies of super-resolution optical microscopy, also known as far-field optical nanoscopy, constitute the future of fluorescence microscopy and bioimaging. This Workshop gathered over 50 graduate students, postdocs and researchers, from various fields such as Biology,
- [Congratulations Prof. Hell for the Nobel Prize in Chemistry!](https://stefani-lab.ar/2014/10/11/congratulations-prof-hell-nobel-prize-chemistry/) - Prof. Dr. Stefan W. Hell has been awarded the Nobel Prize in Chemistry 2014, together with Prof. William E. Moerner and Dr. Eric Betzig. This prize is a well-deserved recognition for Prof. Hell´s career devoted since the early 1990s to find ways of imaging objects optically, with spatial resolution beyond the limit imposed by the
- [¿Qué se premió con el Premio Nobel en Química 2014?](https://stefani-lab.ar/2014/11/01/que-se-premio-con-el-premio-nobel-en-quimica-2014/) - El Dr. Mariano Bossi y el Prof. Dr. Fernando Stefani publicaron en la revista Ciencia Hoy, una reseña sobre el Premio Nobel de Química 2014, otorgado a Stefan W. Hell, William E. Moerner y Eric Betzig por el desarrollo de las nanoscopías de fluorescencia. Dr. Bossi and Prof. Stefani, ambos trabajaron con el Prof. Stefan
- [Applied research award in national innovation contest](https://stefani-lab.ar/2014/11/13/applied-research-award-national-innovations-contest/) - The project BioSeNP presented by Jesica Pellegrotti y Prof. Dr. Fernando Stefani received one of the two top awards in the category Applied Research of the national innovation contest Innovar 2014 organized by the Ministry of Science, Technology and Productive Innovation! BioSeNP consists of a new biosensing platform based on the specific optical heating of
- [Visit of students from Philips technical high-school](https://stefani-lab.ar/2014/11/18/visit-of-students-from-philips-technical-high-school/) - Today we received at CIBION a group of high-school students from the Instituto Tecnológico Philips Argentina motivated for science. We showed them our labs and a typical working day of a researcher. It is always nice to see young students with scientific spirit!
- [Ver para creer - artículo de divulgación sobre microscopía de súper-resolución](https://stefani-lab.ar/2015/08/04/ver-para-creer-articulo-de-divulgacion-sobre-microscopia-de-super-resolucion/) - El Prof. Stefani publicó un artículo de divulgación sobre las técnicas de microscopía de fluorescencia de súper resolución, también conocidas como nanoscopías de fluorescencia, donde describe sus principios de funcionamiento, sus características y capacidades, y la disponibilidad en el país. Leer el artículo "Ver para creer: nanoscopía de fluorescencia"
- [Connecting metallic nanoparticles by optical printing](https://stefani-lab.ar/2016/01/08/connecting-metallic-nanoparticles-by-optical-printing/) - Using optical forces it is possible to "print" colloidal metallic nanoparticles on specific locations of a substrate, directly from the suspension. In this way, it is possible to fabricate arrays of nanoparticles of arbitrary geometry. See for example the map of Argentina made of individual gold nanoparticles of 60 nm in diameter. Of course, it
- [Meeting with our neurobiology collaborators](https://stefani-lab.ar/2016/02/26/reunion-de-trabajo-con-los-grupos-de-los-dres-damian-refojo-y-alfredo-caceres-2/) - Today, we spent all day discussing and brain-storming with our collaborators from the groups of Dr. Damián Refojo (IBIOBA-CONICET, Buenos Aires) and Dr. Alfredo Cáceres (INIMEC-CONICET, Córdoba) We discussed the advances made in our collaborative projects and planned new experiments. In the picture: Raquel Becerra (Ph.D. candidate, Refojo´s group), Catalina von Bilderling (postdoc, Stefani´s group),
- [Visit to production plant and R&D department of Wiener Lab](https://stefani-lab.ar/2016/05/31/visita-a-wiener-lab-en-rosario-2/) - We visited the production plant and R&D department of Wiener-lab in Rosario (Santa Fé, Argentina), as part of a series of meetings where we are evaluating a collaboration to develop a new platform for homogeneous biosensing. In the picture: Catalina von Bilderling (CIBION), Gustavo Capriotti (Wiener), Fernando D. Stefani (CIBION), Andrés Poeylaut (Wiener), Alberto
- [STED at the emission maximum](https://stefani-lab.ar/2016/08/20/sted-emission-maximum/) - In order to obtain super-resolved images, STED nanoscopy uses a laser tuned at the red tail of the emission spectrum of the fluorophore to deplete the excited state by stimulated emission. Since the stimulated emission cross-section follows practically the same dependency with wavelength as the emission, this configuration involves very small cross-sections and therefore high
- [Directional, light-induced growth of nanoparticles](https://stefani-lab.ar/2016/09/20/crecimiento-de-nanoparticulas-direccionado-e-inducido-por-luz/) - Although there are numerous ways reported to induce the growth of nanoparticles with light, very little is known about the role of polarization. Most of the experiments reported so far were done with nanoparticles in suspension, where only average information can be obtained and the role of the polarization of light is impossible to address.
- [A plasmonic Mirage effect misleads single molecule localization](https://stefani-lab.ar/2017/01/20/plasmonic-mirage-effect-misleads-single-molecule-localization/) - Determining the position of single fluorescent molecules is the key concept for all coordinate-stochastic fluorescence nanoscopy methods, such as PALM or STORM, also known as single-molecule localization microscopy (SMLM). Applying these concepts to study nanophotonic devices and interactions between molecules and nanoparticles seems straightforward at first, but it is not! The electromagnetic coupling between the
- [Congratulations Dr. Gargiulo!](https://stefani-lab.ar/2017/03/23/congratulation-dr-gargiulo/) - Today, Julian Gargiulo defended his doctoral thesis "Optical printing of metallic nanoparticles" at the Physics Department of the Faculty of Exact and Natural Sciences, University of Buenos Aires, obtaining the highest honors. Colloidal chemistry provides an enormous variety of nanoparticles, with different shapes, sizes and material composition. These nanomaterials have unique physical and (bio)chemical properties
- [Precision and mechanistic details of optical](https://stefani-lab.ar/2017/09/01/precision-mechanistic-details-optical/) - Optical printing is a wonderful tool to capture colloidal nanoparticles from suspension and place them on specific positions of a substrate. Still, the mechanistic details of the processes involved are complex and not fully understood. In our recent publication in ACS Nano, we provide new insight into the mechanism of optical printing colloidal nanoparticles and
- [Manipulation of nanoscale heat dissipation to improve optical printing](https://stefani-lab.ar/2017/09/17/manipulation-nanoscale-heat-dissipation-improve-optical-printing/) - Optical printing is a wonderful tool to fabricate arrays of colloidal nanoparticles on solid substrates. However, when a nanoparticle absorbs light, it heats up and generates very large temperature gradients. In turn, those temperature gradients produce thermo-phoretic and thermo-osmotic forces that are strong enough to disturb the optical manipulation of nanoparticles at close separation distances.
- [Innovar Award for the automated optical printing of colloidal nanoparticles](https://stefani-lab.ar/2017/10/08/innovar-award/) - The optical manipulation team of our lab was recognized with the Innovar Award 2017 for the method of automated optical printing of colloidal nanoparticles. Congratulations to Santiago Cerrotta, Germán Chiarelli, Cecilia Zaza, Ianina Violi! Of course, also to Julián Gargiulo, who is not in the photo because he is at Imperial College London. Our
- [Dr. Guillermo Acuna visiting professor](https://stefani-lab.ar/2017/10/10/dr-guillermo-acuna-visiting-professor/) - Our close collaborator Dr. Guillermo Acuna from the Technical University of Braunschweig (Germany) was in Buenos Aires, from September 5th to October 7th, as visiting professor of the Faculty of Exact and Natural Sciences of the University of Buenos Aires. Dr. Guillermo Acuna giving a lecture at the Physics Department of FCEN-UBA During his stay,
- [New open-source software for the quantification of protein periodic structures](https://stefani-lab.ar/2017/11/23/new-open-source-software-for-the-quantification-of-protein-periodic-structures-in-fluorescence-nanoscopy-images/) - Fluorescence nanoscopy is enabling the visualization and discovery of periodic protein structures in their natural environment. Remarkable examples are the nuclear pore complex or the membrane-associated periodic cytoskeleton of neurons (MPS). The MPS of neurons can be visualized by fluorescence nanoscopy (e.g. STED) but is invisible using conventional microscopy (e.g. confocal) The next step
- [Book chapter in Neuromethods](https://stefani-lab.ar/2018/03/28/book-chapter-in-neuromethods/) - In the last volume of the Neuromethods series we published a chapter reviewing the use of colloidal gold nanoparticles as nucleation centers for amyloid fibrillation. “Gold Nanoparticles as Nucleation Centers for Amyloid Fibrillation” Yanina D. Álvarez, Jesica V. Pellegrotti, Fernando D. Stefani. Neuromethods 135 (2018) 269-291. Humana Press, New York.
- [We welcome new group members!](https://stefani-lab.ar/2018/04/10/nuevos-integrantes/) - We are very happy to welcome the new group members (from left to right): Germán Chiarelli, PhD candidate, will investigate novel combinations of technologies to obtain fluorescence nanoscopy with sub-10 nm resolution in the three dimensions. Dr. Alan Szalai will focus on deciphering details of neuronal polarity using fluorescence nanoscopy imaging, in collaboration with the
- [Review about the membrane-associated periodic skeleton of neurons](https://stefani-lab.ar/2018/05/23/review-sobre-el-esqueleto-periodico-asociado-a-membrana-de-las-neuronas-2/) - The membrane-associated periodic skeleton (MPS) of neurons is a supramolecular protein structure that we study using fluorescence nanoscopy. Together with our friends, the neurobiologist Dr. Alfredo Cáceres and Dr. Nicolás Unsain, we explain what we know so far about this neuronal nano-structure, what proteins are involved, how are they organized at the molecular level, and
- [Super-resolution imaging at the cell membrane of breast cancer cells](https://stefani-lab.ar/2018/07/08/collaboration-with-dr-marina-simian-article-in-the-journal-of-cell-biology/) - Estrogen receptor α (ERα) is expressed in tissues as diverse as brains and mammary glands. In breast cancer, ERα is a key regulator of tumor progression. Therefore, understanding what activates ERα is critical for cancer treatment in particular and cell biology in general. In this interdisciplinary work, a collaboration led by Dr. Marina
- [MINFLUX: a breakthrough in optical microscopy](https://stefani-lab.ar/2017/01/06/breakthrough-in-optical-microscopy/) - Fluorescence nanoscopy is revolutionizing biological imaging because it keeps all the advantages of fluorescence-based imaging (specificity, sensitivity, low-invasiveness) but provides a spatial resolution no longer limited by diffraction. Although theoretically, all these methods provide unlimited resolution, in practice they are limited by the photostability of fluorophores. The limited photon budget determines the achievable resolution. That
- [MINFLUX as 2017 top-ten breakthrough in Physics](https://stefani-lab.ar/2017/12/12/minflux-as-2017-top-ten-breakthrough/) - Every year, Physics World, the world's leading physics magazine published by the Institute of Physics, selects the 10 major contributions made for the advancement of Physics. We are proud and happy to see that MINFLUX has been included in this short list: 2017 top-ten achievements in Physics according to the Institute of Physics MINFLUX
- [Julián Gargiulo: best doctoral thesis on Experimental Physics of Argentina 2016-2017](https://stefani-lab.ar/2018/09/22/julian-gargiulo-best-doctoral-thesis-on-experimental-physics-of-argentina-2016-2017/) - The Argentine Physics Association (AFA) has elected the thesis of Julián Gargiulo as the best doctoral thesis on Experimental Physics of Argentina for the period 2016-2017. Every year, the AFA awards the Juan José Giambiagi Prize to the best doctoral thesis on Physics carried out in Argentina. Alternately, one year the Prize goes to a
- [Dr. Mariano Barella visits Imperial College London](https://stefani-lab.ar/2018/10/20/dr-mariano-barella-visits-imperial-college-london/) - As part of our collaboration with the group of Prof. Stefan Maier, Dr. Mariano Barella travelled to London for a research stay at Imperial College. During his stay, Mariano will discuss and perform kick-off experiments for a new project with Dr. Julian Gargiulo, an ex-member of our lab currently working at Stefan Maier's lab.
- [Visiting Professor Marcelo Nollmann](https://stefani-lab.ar/2018/10/30/visiting-professor-marcelo-nollmann/) - We are glad to welcome Dr. Marcelo Nollmann from the Center for Structural Biochemistry in Montpellier (CNRS/INSERM) as visiting Professor at the Physics Department of the University of Buenos Aires During his stay, Dr. Nollmann will give a course for graduate and undergraduate students of the Faculty of Exacts and Natural Sciences on "Molecular
- [Asymmetric bifunctional protein nanoparticles through redesign of self-assembly](https://stefani-lab.ar/2019/02/28/asymmetric-bifunctional-protein-nanoparticles-through-redesign-of-self-assembly/) - We have been using DNA self-assembly for a while now. DNA-origami is a wonderful technique to build nanoscopic optical breadboards for our experiments. While DNA is highly versatile, it has the disadvantage of being fluffy... In fact, life uses proteins to build stuff, not DNA. So why not trying to direct protein self-assembly to build
- [Size-Selective Optical Printing of Silicon Nanoparticles](https://stefani-lab.ar/2019/03/18/size-selective-optical-printing-of-silicon-nanoparticles-through-their-dipolar-magnetic-resonance/) - Recently, silicon nanoparticles have raised interest in the plasmonics community they present size-dependent electric and magnetic resonances in the visible range. However, their widespread application has been limited (in comparison to metallic nanoparticles, for example) because their preparation on monodisperse colloids remains challenging. In our recent paper in ACS Photonics, we show that surfactant-free silicon
- [Congratulations Dr. Ponzio!](https://stefani-lab.ar/2019/03/20/felicidades-dr-2/) - Today, Rodrigo Andrés Ponzio defended his doctoral thesis “Energy transfer in nanostructured organic materials” at the Physics Department of the Faculty of Exact and Natural Sciences, University of Buenos Aires, obtaining the highest honors. His thesis was carried out under the supervision of Dr. Fernando Stefani at CIBION and Dr. Rodrigo Palacios at the Institut of Energy research and
- [Research stay of Dr. Florian Steiner from LMU](https://stefani-lab.ar/2019/04/04/visita-del-dr-florian-steiner-2/) - Dr. Florian Steiner, postdoc in Prof. Tinnefeld's group at Ludwig-Maximilians-Universität München, visited our lab during the last month to learn about our new pulsed-interleaved MINFLUX set-up. During his stay, Florian (on the right; are those sunglasses??) worked closely with Ph.D. candidate Luciano Masullo (on the left, with proper laser safety goggles) who is in charge
- [1st Meeting of Young Researchers in Bionanosciences](https://stefani-lab.ar/2019/05/07/1st-meeting-of-young-researchers-in-bionanosciences-2/) - Students and young researchers from various disciplines working in the field of Bionanosciences gathered at the National University of San Martín (UNSAM) to share and discuss their projects, ideas, and perspectives. The meeting was organized by graduate and undergraduate students doing research at CIBION, the National University of San Martín (UNSAM), and the National Atomic
- [XIX Meeting on Surfaces and Nanostructured Materials](https://stefani-lab.ar/2019/06/06/xix-meeting-on-surfaces-and-nanostructured-materials/) - From 5 to 7 June 2019, our national NANO meeting, the XIX Meeting on Surfaces and Nanostructured Materials, was held in Buenos Aires. Since 2001, this event brings together representatives from the academic, scientific and productive sectors to discuss the state of the art, challenges and advances in different areas of nanoscience and nanotechnology. This
- [Luciano Masullo visits the Tinnefeld Lab at LMU](https://stefani-lab.ar/2019/08/05/luciano-masullo-visits-the-tinnefeld-lab-at-lmu-with-a-daad-scholarship-2/) - From August 1st until November 30th, Luciano is visiting the group of Prof. Philip Tinnefeld at the Faculty for Chemistry of the Ludwig-Maximilians-University of Munich (LMU), financed with a DAAD Scholarship. Luciano´s research stay is part of our standing cooperation with the group of Prof. Tinnefeld. He will help our german colleagues building their own
- [Directional Single-Molecule Emission with DNA Origami-Assembled Optical Antennas](https://stefani-lab.ar/2019/08/26/directing-single-molecule-emission-with-dna-origami-assembled-optical-antennas-2/) - DNA-origami is a wonderful technique that enables the fabrications of all kinds of nanoscopic objects. We have been using them to build nanophotonic devices combining metallic nanoparticles to form optical antennas and single molecules. In our recent paper in Nano Letters, we demonstrate how optical nano-antennas self-assembled using DNA-origami can be used to direct the
- [Luciano Masullo presents our new pulsed-interleaved MINFLUX at the PQ meeting](https://stefani-lab.ar/2019/09/05/luciano-masullo-presents-our-new-pulsed-interleaved-minflux-at-the-pq-meeting/) - The PhD project of Luciano Masullo deals with developing a new implementation of MINFLUX using pulsed excitation. He had the great honor of presenting his advances at the special 25th-anniversary edition of the PicoQuant workshop, sharing program with the most brilliant researchers in the field. Prof. Markus Sauer, Prof. Johan Hofkens, Luciano Masullo, Prof.
- [Premio Houssay 2018 to Prof. Stefani](https://stefani-lab.ar/2019/09/13/premio-houssay-2018-to-prof-stefani/) - The National Secretariat of Government for Science, Technology and Productive Innovation awarded the Houssay Award in the category of Mathematics, Physical Sciences, Computer Science and Astronomy to Prof. Dr. Fernando Stefani. The Houssay Award in this category is given once every four years, and is aimed at researchers under 45 who have developed most of
- [Cecilia Zaza wins OSA Emil Wolf Student Paper Competition](https://stefani-lab.ar/2019/09/19/cecilia-zaza-wins-osa-emil-wolf-student-paper-competition-2/) - Each year, the Optical Society of America Foundation recognizes a number of students and early career professionals through different Prizes and Paper Competitions. Cecilia Zaza was recognized with the Emil Wolf Student Paper Competition after presenting her talk on "Size-Selective Optical Printing of Silicon Nanoparticles through Their Dipolar Magnetic Resonance" at the OSA Congress Frontiers
- [Welcome Lars Richter](https://stefani-lab.ar/2019/10/04/welcome-lars-richter-2/) - Lars Richter arrived from Berlin to carry out his Master's thesis in Physics for the Humboldt University of Berlin. Lars obtained a DAAD fellowship to finance his stay and will be working with Luciano Masullo in the new pulsed-interleaved MINFLUX. Welcome Lars and good luck!
- [2018 Houssay Prize in Mathematics, Physics, Computation and Astronomy to Prof. Stefani](https://stefani-lab.ar/2019/11/26/2018-houssay-prize-in-mathematics-physics-computation-and-astronomy-to-prof-stefani/) - In a Ceremony in the Museo del Bicentenario, Prof. Stefani received the 2018 Houssay Prize in Mathematics, Physics, Computation and Astronomy. The Houssay Award is the most important scientific national award granted by the Ministry of Science, Technology, and Innovation. It is aimed at researchers under 45 who have developed most of their scientific activity
- [All set-up for the first Photonics Online Meetup!](https://stefani-lab.ar/2020/01/13/all-set-up-for-the-first-photonics-online-meetup/) - On January 13th, our group participated as a streaming hub in the first edition of the Photonics Online Meetup, an all-virtual, free conference of photonics (https://photonicsonlinemeetup.org/). The conference was entirely online and gathered researchers from all over the world for a one-day meeting that included a poster session via Twitter. An innovative concept for a conference,
- [Quantitative expansion fluorescence microscopy](https://stefani-lab.ar/2020/02/20/quantitative-expansion-fluorescence-microscopy-2/) - Expansion microscopy is a useful alternative to super-resolution imaging to visualize sub-diffraction cellular structures. However, it only works quantitatively if the expansion is calibrated correctly, which is not trivial. In our recent paper in Scientific Reports, led by our collaborator Dr. Nico Unsain, we present a protocol to perform expansion fluorescence microscopy quantitatively. The expansion
- [New equipment for the lab!](https://stefani-lab.ar/2020/03/05/new-equipment-for-the-lab-2/) - We received a shipment with various instruments and equipment coming from Göttingen, as a generous donation of the Max-Planck Society organized by Dr. Thomas Jovin. All the team was very happy and helped with the unloading and unpacking. Thank you so much, Tom, for your friendship and constant support!
- [Detecting fibrillar molecular organization even when it is invisible!](https://stefani-lab.ar/2020/03/25/detecting-molecular-organization-in-sparse-single-molecule-localization-images/) - SMLM is fantastic to obtain super-resolved fluorescence images. However, it is often the case that the density of localizations is too low to obtain a clear image. Instead, sparse distributions of single-molecule positions are obtained, where any underlying molecular organization is invisible to the eye. The reason for this could be an inefficient labeling or
- [Measuring the temperature of a nanoparticle under illumination](https://stefani-lab.ar/2020/09/18/anti-stokes-nanothermometry/) - Plasmonic nanoparticles (NPs) are particularly efficient converters of light into heat, making them useful as remote-controlled nanometric sources of heat in numerous applications. However, measuring the temperature of a NP under illumination is not a simple task. In our recent paper in ACS Nano, we present a nanothermometry method based on anti-Stokes photoluminescence emission. Acquiring
- [A new MINFLUX approach, simpler set-up and with lifetime information!](https://stefani-lab.ar/2020/12/19/a-new-minflux-approach-simpler-set-up-and-with-lifetime-information/) - Since its presentation in early 2017, MINFLUX has generated a lot of interest in the bioimaging community because it enables the localization of single fluorescent molecules with the highest precision, namely 1 nm or less, which is the size of the photon emitting conjugated systems in molecules. However, since then, no other lab in the
- [Orienting single-molecules in DNA-origami](https://stefani-lab.ar/2021/03/04/orienting-single-molecules-in-dna-origami/) - DNA-origami technology is wonderful to fabricate all kinds of nanoscopic objects by self-assembly. We, in particular, use them to fabricate nanophotonic devices. That involves placing photon emitters alongside nanoparticles of different materials on well-defined nanometric geometries. DNA-origami is unique in the sense they provide great positional and stoichiometric control; we can decide where to place
- [Finally! Super-resolved FRET imaging](https://stefani-lab.ar/2021/02/24/finally-super-resolved-fret-imaging/) - Förster resonance energy transfer (FRET) from a fluorophore in the excited state (donor, D) to a fluorophore in the ground state (acceptor, A) provides a signal sensitive to D−A separation distances in the 0−10 nm range, and to various environmental parameters. A vast number of FRET imaging protocols and sensors have been developed and applied
- [Congratulations Lars!](https://stefani-lab.ar/2020/10/05/congrats-lars/) - Lars Richter came to our group in October 2019 with the support of a DAAD fellowship. For his Master's thesis, he worked closely with Luciano Masullo on our pulsed-interleaved MINFLUX, and was a great member of our research team. Now, Lars has moved to Munich to start a Ph.D. program under the supervision of Prof.
- [Summer festival meeting with Prof. Hell](https://stefani-lab.ar/2017/08/03/summer-festival-meeting-prof-hell/) - During the summer festival of the Max-Planck-Institute for Biophysical Chemistry, we had the chance to discuss with Stefan about the impact and future development of MINFLUX. Thank you Stefan for the constant support!
- [Congratulations to new Physicist Alfredo Sánchez](https://stefani-lab.ar/2015/11/12/congratulations-new-physicist-alfredo-sanchez/) - Today, Alfredo Sánchez obtained his degree on Physics at the University of Buenos Aires, defending his undergrad thesis on Preparation and optical properties of gols nanorods coated with silica and organic fluorophores. Congratulations Alfredo!
- [Congratulations to new Physicist Santiago Cerrotta](https://stefani-lab.ar/2015/12/16/congratulations-new-phycisist-santiago-cerrotta/) - Today, Santiago Cerrotta obtained his degree on Physics with the highest honors, defending his undergraduate thesis on automated optical printing of gold and silver nanoparticles. Congratulations Santi!
- [OSA Student Chapter - 2nd Metting of Young Researchers in Optics and Photophysics](https://stefani-lab.ar/2016/12/02/3718/) - After the success of the first meeting, CIBION hosted the 2nd Metting of Young Researchers in Optics and Photophysics sponsored by the Optical Society of America through its Student Chapter. Again, it was a great success. Congratulations to the local organizers and all participants. We look forward to the next meeting!
- [Georg Foster Research Award to Prof. Fernando Stefani](https://stefani-lab.ar/2017/07/04/georg-foster-research-award-prof-fernando-stefani/) - The Georg Forster Research Award is granted by the prestigious Alexander von Humboldt Foundation (AvH) in recognition of a researcher's entire achievements to date to academics of all disciplines whose fundamental discoveries, new theories, or insights have had a significant impact on their own discipline and beyond and who are expected to continue developing research-based solutions
- [Se reduce nuevamente el presupuesto para Ciencia y Tecnología](https://stefani-lab.ar/2017/11/21/3710/) - Es importante que los miembros de la comunidad científica y sus socios industriales conozcan la evolución de la inversión pública en el sistema científico-tecnológico. El proyecto de ley de presupuesto enviado por el Poder Ejecutivo Nacional (PEN) plantea una nueva reducción de la inversión pública en ciencia y técnica. Para 2018 se propone una reducción
- [Prof. Stefani receives the Georg-Foster Research Award of the Alexander von Humboldt Foundation](https://stefani-lab.ar/2018/04/16/prof-stefani-receives-georg-foster-research-award-alexander-von-humboldt-foundation/) - Every year, the Alexander von Humboldt foundation honors researchers from all over the world and all disciplines with its prestigious Research Awards. This year, 4 argentine researchers were among the 45 awardees:Dra. Vanesa Gottifredi, Prof. Dr. Carlos Ballare, Prof. Dr. Daniel De Florian, and Prof. Dr. Fernando Stefani. The award ceremony was held during the
- [Entrevista al Prof. Stefani: Microscopía de súper-resolución en Argentina](https://stefani-lab.ar/2013/11/06/microscopia-de-super-resolucion-en-argentina/) - Entrevista al Prof. Dr. Fernando Stefani sobre la aplicación de microscopía de super-resolución en Argentina https://www.mincyt.gob.ar/destacado/una-mirada-diferente-9060
- [Setting up the new optics lab @CIBION](https://stefani-lab.ar/2014/04/29/setting-up-the-optics-lab/) - We are moving our labs from the Faculty of Exact and Natural Sciences of the University of Buenos Aires to CIBION. We have just received the first instruments and are very happy that we can start setting up the new lab. Here a sequence of the first steps setting up the new lab! August 2013
- [DINAMO: Discussion on Nano and Mesoscopic Optics](https://stefani-lab.ar/2014/10/10/dinamo-discussions-nano-mesoscopic-optics/) - Nano and Mesoscopic Optics involves the interaction of light with structures that are too small or too complex to be described by traditional continuum methods, as well as the structuring and manipulation of optical fields and interactions at sub-wavelength scale. This is a highly active and interdisciplinary area of research which involves experimental and theoretical
- [Award of National Academy to Prof. Stefani](https://stefani-lab.ar/2014/10/12/award-of-the-national-academy-for-prof-stefani/) - Prof. Stefani has been recognized with the award "Mauel Cardona" in Physics of the National Academy of Exact, Physical and Natural Sciences (ANCEFN). ANCEFN website
- [DINAMO conference in Patagonia, a tremendous success!](https://stefani-lab.ar/2015/04/15/dinamo-conference-in-patagonia-a-tremendous-success/) - DINAMO, Discussions on Nano and Mesoscopic Optics, was launched to promote the transfer of knowledge between different branches of science and stimulate global solutions to complex problems of nano and mesoscopic optics. DINAMO is conceived to provide an interdisciplinary forum where leading investigators from around the world can present and discuss their latest work and
- [Completion of the Polo Científico Tecnológico ex Bodegas Giol](https://stefani-lab.ar/2015/11/06/completion-of-the-polo-cientifico-tecnologico-ex-bodegas-giol/) - With the inauguration of the CONICET building, the Science Cultural Center (Centro Cultural de la Ciencia - C3), and the Science Park, the "Polo Científico Tecnológico ex Bodegas Giol", where CIBION is located, has been completed! Science Park Today is a happy and memorable day for the whole scientific community of Argentina. The "Polo"
- [Academic Excellence Honor to Prof. Stefani](https://stefani-lab.ar/2015/11/19/academic-excellence/) - Prof. Stefani has received the Honorable Mention to Academic Excellence of the University of Buenos Aires.
- [Visiting Professor Philip Tinnefeld](https://stefani-lab.ar/2015/12/16/visiting-professor-philip-tinnefeld/) - Prof. Dr. Philip Tinnefeld from the Technical University of Braunschweig (Germany) will spend 3 months with us as a visiting researcher of CONICET. Philip is a friend and one of our closest collaborators. He just arrived for the Physics Department bbq. Welcome, Philip!
- [OSA Student Chapter - 1st Meeting Young Researchers in Optics and Photophysics of Argentina](https://stefani-lab.ar/2016/05/26/osa-student-chapter-1st-meeting-young-researchers-in-optics-and-photophysics-of-argentina/) - CIBION hosted the first meeting of Young Researchers in Optics and Photophysics of Argentina sponsored by the Optical Society of America (OSA) through its Student Chapter. It was a day full of enthusiastic and interesting talks and discussions. Thank you Cecilia Zaza for the local organization.
- [Presupuesto MINCYT, ANPCYT, CONICET, CONAE](https://stefani-lab.ar/2016/10/03/presupuesto-mincyt-anpcyt-conicet-conae-2/) - Es importante que la comunidad científica, y sus socios industriales, conozcan la evolución de la inversión pública en el sistema científico. Lamentablemente, el proyecto de ley de presupuesto enviado por el Poder Ejecutivo Nacional impone importantes recortes para el año próximo. Los invito a leer, comentar y ampliar estos estudio: 2016 - Magnitud y Consecuencias
- [Congratulations Dr. Bordenave!](https://stefani-lab.ar/2016/11/14/congratulations-dr-bordenave/) - Today, Martin Diego Bordenave defended his PhD thesis "Mediciones con resolución nanométrica mediante depleción por emisión estimulada" at the Physics Department of the Faculty of Exact and Natural Sciences, University of Buenos Aires, obtaining the highest honors. In his thesis, Martín built and applied two STED nanoscopes: - firstly, he built a multicolor STED
- [OSA Student Chapter - 2nd Metting of Young Researchers in Optics and Photophysics](https://stefani-lab.ar/2017/12/02/osa-student-chapter-2nd-metting-of-young-researchers-in-optics-and-photophysics-2/) - After the success of the first meeting, CIBION hosted the 2nd Metting of Young Researchers in Optics and Photophysics sponsored by the Optical Society of America through its Student Chapter. Again, it was a great success. Congratulations to the local organizers and all participants. We look forward to the next meeting!
- [Visiting Professor Thomas Klar](https://stefani-lab.ar/2016/12/10/visiting-professor-thomas-klar/) - We are happy to receive Prof. Dr. Thomas Klar as a visiting researcher of CONICET. He will stay with us for 3 months. Following the steps of Philip Tinnefeld, he also arrived just in time for the famous Physics Department bbq! Welcome, Thomas!
- [Congratulations Dr. Barabas!](https://stefani-lab.ar/2017/03/23/congratulations-dr-barabas/) - Today, Federico Barabas defended his doctoral thesis "Nanoscopía de fluorescencia por localización estocástica de moléculas individuales" at the Physics Department of the Faculty of Exact and Natural Sciences, University of Buenos Aires, obtaining the highest honors. We highly appreciate the evaluation by the jury: Prof. Dr. Francisco Barrantes, Prof. Dr. Hernán Grecco, and Prof. Dr.
- [DINAMO 2017 in Siglufjordur, Iceland](https://stefani-lab.ar/2017/05/22/dinamo-2017-siglufjordur-iceland/) - The conference Discussions on Nano and Mesoscopic Optics (DINAMO) aims to promote the transfer of knowledge between different branches of optical sciences and to stimulate global solutions to complex questions nano- and mesosopic optics. DINAMO is conceived to provide an interdisciplinary forum where leading scientists from around the world could present and discuss their latest
- [Happy 60th birthday Niek van Hulst!](https://stefani-lab.ar/2017/10/21/happy-60th-birthday-niek-van-hulst/) - On friday 20th of October, friends and colleagues of Prof. Dr. Niek van Hulst came together from around the globe, to celebrate his 60th birthday discussing some of Niek´s favorite topics in Nanophotonics: Manipulation and control of molecular beams Precision spectroscopy of molecular hydrogen and the search for new physics Light twists at the nanoscale
- [Dr. Ianina Violi new member of CONICET](https://stefani-lab.ar/2017/12/29/dr-ianina-violi-new-member-of-conicet/) - Dr. Ianina Violi has been elected to join the National Scientific and Technical Research Council (CONICET) as Assistant Researcher, directed by Dr. Galo Soler Illia and co-directed by Prof. Fernando D. Stefani. On a very competitive contest, Ianina was one of the 17 new researchers selected to join CONICET in Chemistry. In her new role,
- [Congratulations to Physicist Germán Chiarelli](https://stefani-lab.ar/2018/02/28/congratulations-to-physicist-german-chiarelli/) - German Chiarelli obtained his degree in Physics at the University of Buenos Aires with his work on "Optical printing of colloidal nanoparticles in a microfluidic cell" Congratulations Germán!!
- [1st Summer School on Photothermal Effects in Plasmonics](https://stefani-lab.ar/2018/06/25/1st-summer-school-on-photothermal-effects-in-plasmonics-2/) - Dr. Ianina Violi and Cecilia Zaza obtained a travel grant to attend the 1st Summer School on Photothermal Effects in Plasmonics, taking place in Porquerolles’ Island, France, and they made the sacrifice to represent the group... The school gathered more than 60 researchers from around the world, including our former mate Julián Gargiulo!
- [8th Single Molecule Localization Microscopy Symposium](https://stefani-lab.ar/2018/08/27/8th-single-molecule-localization-microscopy-symposium-2/) - Cecilia Zaza and Luciano Masullo participated in the 8th Single Molecule Localization Microscopy Symposium, presenting the software Gollum for the automated quantification of periodic protein structures The meeting took place at the beautiful Harnack Haus of the Max-Planck Society in Berlin, Germany. More information about the symposium at https://2018.smlms.org/ More info about the work: Federico M.
- [11ᵗʰ Advanced Scientific Programming in Python](https://stefani-lab.ar/2018/09/05/11ᵗʰ-advanced-scientific-programming-in-python-2/) - Luciano Masullo participated in the 11ᵗʰ Advanced Scientific Programming in Python, taking place in Camerino, Italy, from 3rd to 8th of September The school focused on advanced Python programming for scientific application and was aimed at scientific investigators, not professional programmers. Among other topics, the participants learned strategies and methods to develop and test projects,
- [Dr. Ianina Violi explains nanotechnology on open TV](https://stefani-lab.ar/2019/10/20/dr-ianina-violi-explains-nanotechnology-on-open-tv/) - Using examples from our lab, Dr. Ianina Violi explains the essence of nanotechnology on public television. Looking good on TV Iani!

## Pages

- [Home](https://stefani-lab.ar/) - Center for Bionanoscience Research (CIBION), National Scientific and Technical Research Council (CONICET) Physics Department, Faculty of Exact and Natural Sciences, University of Buenos Aires (UBA) Using optical methods, we explore the properties and technological applications of nanoparticles, single molecules, nanostructured materials, supramolecular assemblies, biological and hybrid nanosystems. The Applied nanoPhysics Group was launched in October
- [Docencia](https://stefani-lab.ar/docencia/) - Docencia Laboratorio 3 – Laboratorio 4 – Laboratorio 5 La física, al igual que todas las ciencias, avanza en una retroalimentación positiva con la tecnología. Las teorías físicas se ponen a prueba, se confirman o se refutan, en base a experimentos. Los avances científicos permiten el desarrollo de nuevas tecnologías, las nuevas tecnologías habilitan nuevos
- [Fernando D. Stefani](https://stefani-lab.ar/stefani/) - DOWNLOAD CV Stefani CV – esp – 2025_10 Stefani CV – eng – 2025_10 CONTACT Centro de Investigaciones en Bionanociencias (CIBION, CONICET) Godoy Cruz 2390, C1425FQD Buenos Aires, Argentina T: +54 11 4899 5590 E: fernando.stefani@df.uba.ar W: https://stefani-lab.ar/ FERNANDO D. STEFANI FIELDS OF INTEREST AND EXPERTISE Nanoscale manipulation of light, heat and matter Super-resolution fluorescence
- [Publications](https://stefani-lab.ar/publications/) - Publications 2025 Florencia Edorna, Florencia D. Choque, Giovanni Ferrari, Luciano A. Masullo, Piotr Zdańkowski, Guillermo P. Acuna, Philip Tinnefeld, Alan M. Szalai, Lucía F. Lopez, Andrés Zelcer & Fernando D. Stefani “Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy” Light: Science & Applications 14 (2025) 385 Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey,
- [FOMISUR 2025](https://stefani-lab.ar/fomisur-2025/) - RED FEDERAL DE MICROSCOPÍA DE SUPER-RESOLUCIÓN Día 1 Clases sobre principios de funcionamiento, protocolos de marcación y medición Día 2 Experimentos de STED y SMLM en los nanoscopios de CIBION – Propuestas de proyectos por participantes Día 3 Conferencias por especialistas invitados sobre aplicaciones de super-resolución Día 4 Conferencias por especialistas
- [FOMISUR 2024](https://stefani-lab.ar/fomisur-2024/) - RED FEDERAL DE MICROSCOPÍA DE SUPER-RESOLUCIÓN Día 1 Clases sobre principios de funcionamiento, protocolos de marcación y medición Día 2 Demostración de experimentos de STED y SMLM en los nanoscopios de CIBION Día 3 Conferencias por especialistas invitados sobre aplicaciones de super-resolución Día 4 Discusión de proyectos propuestos por participantes y posibles colaboraciones CONFERENCISTAS
- [Política Científica](https://stefani-lab.ar/politica-cientifica/) - Política científica En este espacio publico mis análisis y reflexiones sobre el sistema científico nacional, en particular sobre el rol de la ciencia y la innovación industrial deben tener para impulsar el desarrollo del país. Todos los comentarios son bienvenidos por correo electrónico. ¿Para qué sirve la ciencia en términos económicos? ¿Cómo se pasa de
- [Group pictures](https://stefani-lab.ar/group-pictures/) - GROUP PICTURES 2010 Fernando Diaz, Fernando Stefani, Andrés Benassi, Jesica Pellegrotti 2011 Fernando Stefani, Andrés Benassi, Jesica Pellegrotti, Fernando Diaz 2013 Fernando Stefani, Emiliano Cortés, Yanina Alvarez, Federico Barabas, Julián Gargiulo, Jesica Pellegrotti (Martin Bordenave not in the picture since he was in Göttingen making a stay in the group of Stefan Hell). 2014 Julián
- [News](https://stefani-lab.ar/news/) - News Prof. Fernando D. Stefani Joins the Editorial Board of Light: Science & Applications nano_x7n4r02026-01-22T16:47:00+00:00December 1st, 2025| We are pleased to announce that Prof. Fernando D. Stefani has been appointed as [...] Read More Open-source sub-nanometer stabilization system for super-resolution microscopes nano_x7n4r02026-01-22T16:20:15+00:00November 21st, 2025| All optical microscopes, regardless of how well they are constructed, exhibit

## Portfolio

- [General Practice](https://stefani-lab.ar/portfolio-items/general-practice/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden
- [Cardiology](https://stefani-lab.ar/portfolio-items/cardiology/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden
- [Pediatrics](https://stefani-lab.ar/portfolio-items/pediactrics/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden
- [Diabetes Care](https://stefani-lab.ar/portfolio-items/diabetes-care/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden
- [Pre-natal Care](https://stefani-lab.ar/portfolio-items/pre-natal-care/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden
- [Ultrasound Echocardiogram](https://stefani-lab.ar/portfolio-items/ultrasound-echocardiogram/) - Expert Health Unit There are many variations of passages of Lorem Ipsum available, but the majority have suffered alteration in some form, by injected humour, or randomised words which don’t look even slightly believable. If you are going to use a passage of lorem ipsum you need to be sure there isn’t anything embarrassing hidden

## Categories

- [Uncategorized](https://stefani-lab.ar/category/uncategorized/)
- [english](https://stefani-lab.ar/category/english/)

## Portfolio Categories

- [Cardiology](https://stefani-lab.ar/portfolio_category/cardiology/)
- [Care](https://stefani-lab.ar/portfolio_category/care/)
- [General Practice](https://stefani-lab.ar/portfolio_category/general-practice/)
- [Pediactrics](https://stefani-lab.ar/portfolio_category/pediactrics/)
- [Sports](https://stefani-lab.ar/portfolio_category/sports/)
- [Ultrasound](https://stefani-lab.ar/portfolio_category/ultrasound/)