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Center for Bionanoscience Research (CIBION), National Scientific and Technical Research Council (CONICET)
Physics Department, Faculty of Exact and Natural Sciences, University of Buenos Aires (UBA)

 Using optical methods, we explore the properties and technological applications of nanoparticles, single molecules, nanostructured materials, supramolecular assemblies, biological and hybrid nanosystems.

The Applied nanoPhysics Group was launched in October 2009 at the Physics Department, Faculty of Exact and Natural Sciences,  of the University of Buenos Aires (UBA)

In 2012 we moved our labs to the Center for Bionanoscience Research (CIBION) of the National Scientific and Technical Research Council (CONICET), while Prof. Stefani still holds his position at the Physics Department of the University of Buenos Aires.

CURRENT RESEARCH ACTIVITIES

Fluorescence nanoscopy.

Super-resolution fluorescence microscopy, also known as fluorescence nanoscopy, has revolutionized biological imaging because they provide deep sub-wavelenght spatial resolution while keeping the low-invasiveness of far-field optical interrogation. We apply and optimize well-established methods like STED and STORM, and develop new ones, such as MINFLUX, and open-source software for fluorescence nanoscopy. With them, we address questions of cellular and neuronal biology.

Optical printing colloidal nanoparticles.

Colloidal chemistry enables the fabrication of nanoparticles of different shapes, sizes and material compositions, that exhibit unique physical and chemical properties, inexistent in bulk materials. In order to make use of those properties in devices and circuits, it is necessary to develope methods to bring the colloidal nanoparticles from the liquid phase to specific locations of solid substrates. We address this challenge using optical forces.

Self-assembled Nanophotonic Devices.

Semiconductor-based devices are approaching intrinsic limits of speed and heat dissipation.
Optical devices are faster and practically loss-less, but their size miniaturization is limited by the wavelength of light. Nanophotonics and Plasmonics deals with the manipulation of light at the nanoscale. We investigate light-matter interaction between single-photon emitters and metallic nanoparticles organized in nanodevices by self-assembly.

LATESTS NEWS

Presentations a the annual meeting of the Argentine Society of Biophysics (SAB 2022)

November 18th, 2022|

This year, the annual meeting of the SAB was held in Rosario. CIBION contributed with the folowing presentations: Gonzalo Escalante - "Super-resolution of membrane proteins in Trypanosoma cruzi" Santiago Sosa - "Determining the orientation of single molecules in a protein cage by fluorescence nanoscopy" Luis Marcano-García - "Determination of [...]

Interviews to Luciano Masullo, Lucía Lopez and Fernando Stefani

November 3rd, 2022|

Focal Plane has made a series of interviews to  latin american microscopy scientists. Three members of our lab were interviewed to tell about their experiences:   An interview with Fernando Stefani - FocalPlane (biologists.com)   An interview with Lucía López - FocalPlane (biologists.com)   An interview with Luciano Masullo - [...]

Prof. Stefani visits friends and collaborators in Munich

September 20th, 2022|

During three weeks in September, Prof. Stefani visited friends and collaborators en the Munich area. He gave lectures and supervised ongoing projects in collaboration. Prof. Stefani visited: Prof. Philip Tinnefeld and his group at the Faculty for Chemistry and Pharmacy of the LMU Munich, where former members [...]

Assembling single molecules with controlled orientation

September 7th, 2022|

An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control and well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up nanofabrication methodology that fulfils almost all of these features. It enables the nanometric positioning of molecules and nanoparticles [...]

New laser thanks to the Alexander von Humboldt Foundation!

August 24th, 2022|

The Alexander von Humboldt Foundation has granted us an Equipment Subsidy to purchase a new supercontinuum laser that will be used for future pulsed-MINFLUX experiments.   Ph. D. candidate Florencia Choque already set it up and running. Thanks so much to the Humboldt Foundation for this great support!  

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THE GROUP

PROF. DR. FERNANDO D. STEFANI
PROF. DR. FERNANDO D. STEFANIPrincipal Investigator
DR. LUCÍA LÓPEZ
DR. LUCÍA LÓPEZLab Manager
Dr. ALAN SZALAI
Dr. ALAN SZALAIAssistant Researcher
Super-resolution fluorescence microscpy, Single-molecule biophysics
DR. IANINA L. VIOLI
DR. IANINA L. VIOLIAssistant Researcher (INS-UNSAM, CIBION)
Synthesis, modification and optical manipulation of colloidal nanoprticles
DR. JULIÁN GARGIULO
DR. JULIÁN GARGIULOAssistant Researcher (INS-UNSAM, CIBION)
Optical printing colloidal nanoparticles. Plasmon-assisted chemistry.
LUCIANA MARTÍNEZ
LUCIANA MARTÍNEZPh.D. candidate in Physics (University of Buenos Aires)
Plasmon-assisted chemistry
SANTIAGO SOSA
SANTIAGO SOSAPh.D. candidate in Biological Chemistry (University of Buenos Aires)
Nanofabrication by protein self-assembly
GONZALO ESCALANTE
GONZALO ESCALANTEPh.D. candidate in Physics (University of Buenos Aires)
Super-resolution imaging of chromatin
FLORENCIA CHOQUE
FLORENCIA CHOQUEPh.D. candidate in Physics (University of Buenos Aires)
pulsed interleaved MINFLUX
FLORENCIA EDORNA
FLORENCIA EDORNAPh.D. candidate in Physics (University of Buenos Aires)
Single-molecule localization through sequential structured illumination
GROUP PICTURES

COLLABORATORS

STEFAN HELL
Max-Planck-Institute for Biophysical Chemistry (Göttingen, Germany)

THOMAS JOVIN
Max-Planck-Institute for Biophysical Chemistry (Göttingen, Germany)

ALFREDO CÁCERES
Instituto Universitario de Ciencias Biomédidas de Córdoba (Córdoba, Argentina)

PHILIP TINNEFELD
Ludwig-Maximilians-University Munich (Germany)

GUILLERMO ACUNA
University of Fribourg (Switzerland)

STEFAN MAIER
Ludwig-Maximilians-University Munich (Germany)

ANDREA BRAGAS
University of Buenos Aires (Argentina)

JOCHEN FELDMANN
Ludwig-Maximilians-University Munich (Germany)

NIEK VAN HULST
Institute of Photonic Sciences (Barcelona, Spain)

EDUARDO CORONADO
Universidad Nacional de Córdoba (Córdoba, Argentina)

SABRINA SIMONCELLI
University College London (UK)

DAMIAN REFOJO
Biomedicine Research Institute of Buenos Aires (Argentina)

RODRIGO PALACIOS
Universidad Nacional de Río Cuarto (Córdoba, Argentina)

OSCAR CAMPETELLA
Universidad Nacional de General San Martín (Buenos Aires, Argentina)

OMAR AZZARONI
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (La Plata, Buenos Aires, Argentina)

GALO SOLER ILLIA
Universidad Nacional de General San Martín (Buenos Aires, Argentina)

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