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Center for Bionanoscience Research (CIBION), National Scientific and Technical Research Council (CONICET)
Physics Department, Faculty of Exact and Natural Sciences, University of Buenos Aires (UBA)

 Using optical methods, we explore the properties and technological applications of nanoparticles, single molecules, nanostructured materials, supramolecular assemblies, biological and hybrid nanosystems.

The Applied nanoPhysics Group was launched in October 2009 at the Physics Department, Faculty of Exact and Natural Sciences,  of the University of Buenos Aires (UBA)

In 2012 we moved our labs to the Center for Bionanoscience Research (CIBION) of the National Scientific and Technical Research Council (CONICET), while Prof. Stefani still holds his position at the Physics Department of the University of Buenos Aires.

CURRENT RESEARCH ACTIVITIES

Fluorescence nanoscopy.

Super-resolution fluorescence microscopy, also known as fluorescence nanoscopy, has revolutionized biological imaging because they provide deep sub-wavelenght spatial resolution while keeping the low-invasiveness of far-field optical interrogation. We apply and optimize well-established methods like STED and STORM, and develop new ones, such as MINFLUX, and open-source software for fluorescence nanoscopy. With them, we address questions of cellular and neuronal biology.

Optical printing colloidal nanoparticles.

Colloidal chemistry enables the fabrication of nanoparticles of different shapes, sizes and material compositions, that exhibit unique physical and chemical properties, inexistent in bulk materials. In order to make use of those properties in devices and circuits, it is necessary to develope methods to bring the colloidal nanoparticles from the liquid phase to specific locations of solid substrates. We address this challenge using optical forces.

Self-assembled Nanophotonic Devices.

Semiconductor-based devices are approaching intrinsic limits of speed and heat dissipation.
Optical devices are faster and practically loss-less, but their size miniaturization is limited by the wavelength of light. Nanophotonics and Plasmonics deals with the manipulation of light at the nanoscale. We investigate light-matter interaction between single-photon emitters and metallic nanoparticles organized in nanodevices by self-assembly.

LATESTS NEWS

Assembling single molecules with controlled orientation

September 7th, 2022|

An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control and well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up nanofabrication methodology that fulfils almost all of these features. It enables the nanometric positioning of molecules and nanoparticles [...]

New laser thanks to the Alexander von Humboldt Foundation!

August 24th, 2022|

The Alexander von Humboldt Foundation has granted us an Equipment Subsidy to purchase a new supercontinuum laser that will be used for future pulsed-MINFLUX experiments.   Ph. D. candidate Florencia Choque already set it up and running. Thanks so much to the Humboldt Foundation for this great support!  

Back to in-person meetings! Day of the Physics Department

August 16th, 2022|

We are very happy that the traditional annual get together of the Physics Department is back, and that we can discuss science and education face-to-face with our colleagues and friends!     The Day of the Physics Department (Día del DF, DDF) is a great meeting where professors, researchers, graduate [...]

Ultracompact and unidirectional optical nano antennas

July 31st, 2022|

By nature, photon emission occurs without directionality. In most situations photon emission is dipolar, and multipolar emission may be obtained under very special conditions. Therefore, imposing directionality requires the mediation of specific devices. Directional optical nano antennas have been demonstrated over a decade ago using the Yagi-Uda concept; i.e. a [...]

Happy birthday Prof. Jochen Feldmann!

July 17th, 2022|

Prof. Jochen Feldmann has been a great scientist and mentor for many younger researchers. On the occasion of his 60th birthday, several of his mentorees and collaborators have contributed to a special issue of Advances Optical Materials in his honor.   Happy birthday Jochen!!!   Advanced Optical Materiales - Special [...]

Visit to our collaborator Prof. Guillermo Acuna

June 30th, 2022|

During two weeks of June, Prof. Stefani visited our close collaborator Prof. Guillermo Acuna at the University of Fribourg, Switzerland, where several former members of our group are currently working, like Dr. Mariano Barella and Ph.D. candidate Germán Chiarelli. Prof. Stefani gave lectures on the recent advances of our [...]

An alternative to MINFLUX that enables nanometre resolution in a confocal microscope

June 1st, 2022|

Fluorescence microscopy is a major workhorse in life sciences, biophysics, and physical chemistry, as it allows visualization with great specificity and sensitivity. In particular, the detection of single fluorescent molecules has been used to provide information beyond ensemble averages with applications in different fields of research. For example, super-resolution methods [...]

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THE GROUP

PROF. FERNANDO D. STEFANI
PROF. FERNANDO D. STEFANIPrincipal Investigator
DR. LUCÍA LÓPEZ
DR. LUCÍA LÓPEZStaff Researcher and Lab Manager
Dr. ALAN SZALAI
Dr. ALAN SZALAIAssistant Researcher
Super-resolution fluorescence microscpy, Single-molecule biophysics
DR. JULIÁN GARGIULO
DR. JULIÁN GARGIULOAssistant Researcher (INS-UNSAM, CIBION)
Optical printing colloidal nanoparticles. Plasmon-assisted chemistry.
FLORIAN HABERKORN WAIGEL
FLORIAN HABERKORN WAIGELPh.D. candidate in Physics (University of Buenos Aires)
Single-particle tracking with p-MINFLUX
FACUNDO ROUQUAUD
FACUNDO ROUQUAUDUndergraduate student in Physics (University of Buenos Aires)
pulsed-interleavead STED-FRET
GONZALO ESCALANTE
GONZALO ESCALANTEPh.D. candidate in Physics (University of Buenos Aires)
Super-resolution imaging of protein organization
FLORENCIA CHOQUE
FLORENCIA CHOQUEPh.D. candidate in Physics (University of Buenos Aires)
pulsed interleaved MINFLUX
FLORENCIA EDORNA
FLORENCIA EDORNAPh.D. candidate in Physics (University of Buenos Aires)
Single-molecule localization through sequential structured illumination
LOURDES SIMÓ
LOURDES SIMÓUndergraduate student in Physics (University of Buenos Aires)
3D super-resolution imaging
GROUP PICTURES

COLLABORATORS

STEFAN HELL
Max-Planck-Institute for Biophysical Chemistry (Göttingen, Germany)

THOMAS JOVIN
Max-Planck-Institute for Biophysical Chemistry (Göttingen, Germany)

ALFREDO CÁCERES
Instituto Universitario de Ciencias Biomédidas de Córdoba (Córdoba, Argentina)

PHILIP TINNEFELD
Ludwig-Maximilians-University Munich (Germany)

GUILLERMO ACUNA
University of Fribourg (Switzerland)

ANDRÉS ZELCER
Centro de Investigaciones en Bionanociencias (Buenos Aires, Argentina)

ANDREA BRAGAS
University of Buenos Aires (Argentina)

DARÍO KRAPF
Instituto de Biología Molecular y Celular de Rosario (IBR – Santa Fe, Argentina)

SABRINA SIMONCELLI
University College London (UK)

DAMIAN REFOJO
Biomedicine Research Institute of Buenos Aires (Argentina)

RODRIGO PALACIOS
Universidad Nacional de Río Cuarto (Córdoba, Argentina)

OSCAR CAMPETELLA
Universidad Nacional de General San Martín (Buenos Aires, Argentina)

SPONSORS

We highly appreciate the support by

CONTACT

Centro de Investigaciones en Bionanociencias (CIBION, CONICET)

Godoy Cruz 2390 – Piso 1

C1425FQD Buenos Aires, Argentina

T: +54 11 4899-5500 int. 5596

E: cibion@cibion.conicet.gov.ar

W: https://stefani-lab.ar/

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