Many physical, chemical, and biological phenomena are determined by dynamical processes occurring at the nanoscale. Therefore, tracking
(bio)molecules and nanoparticles with nanometric precision and high temporal resolution is a truly powerful discovery tool in various fields.
When it comes to biology, tracking the motion and conformational changes of proteins lies at the core of deciphering the mechanisms of
life and diseases.
In this article, Prof. Stefani discusses the suitability of optical methods for single molecule and single particle tracking in soft-matter and biological systems. In particular, based on the conceptual and experimental advances of recent years, he presents the advantages of using localization methods based on a minimum of light intensity.
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Fernando D. Stefani
