Determining the position of single fluorescent molecules is the key concept for all coordinate-stochastic fluorescence nanoscopy methods, such as PALM or STORM, also known as single-molecule localization microscopy (SMLM). Applying these concepts to study nanophotonic devices and interactions between molecules and nanoparticles seems straightforward at first, but it is not! [...]

Fluorescence nanoscopy is revolutionizing biological imaging because it keeps all the advantages of fluorescence-based imaging (specificity, sensitivity, low-invasiveness) but provides a spatial resolution no longer limited by diffraction. Although theoretically, all these methods provide unlimited resolution, in practice they are limited by the photostability of fluorophores. The limited photon budget [...]

We are happy to receive Prof. Dr. Thomas Klar as a visiting researcher of CONICET. He will stay with us for 3 months. Following the steps of Philip Tinnefeld, he also arrived just in time for the famous Physics Department bbq! Welcome, Thomas!

After the success of the first meeting, CIBION hosted the 2nd Metting of Young Researchers in Optics and Photophysics sponsored by the Optical Society of America through its Student Chapter. Again, it was a great success. Congratulations to the local organizers and all participants.   We look forward to the [...]

Today, Martin Diego Bordenave defended his PhD thesis "Mediciones con resolución nanométrica mediante depleción por emisión estimulada" at the Physics Department of the Faculty of Exact and Natural Sciences, University of Buenos Aires, obtaining the highest honors.   In his thesis, Martín built and applied two STED nanoscopes: - firstly, [...]

Es importante que la comunidad científica, y sus socios industriales, conozcan la evolución de la inversión pública en el sistema científico. Lamentablemente, el proyecto de ley de presupuesto enviado por el Poder Ejecutivo Nacional impone importantes recortes para el año próximo. Los invito a leer, comentar y ampliar estos estudio: [...]

Although there are numerous ways reported to induce the growth of nanoparticles with light, very little is known about the role of polarization. Most of the experiments reported so far were done with nanoparticles in suspension, where only average information can be obtained and the role of the polarization of [...]

In order to obtain super-resolved images, STED nanoscopy uses a laser tuned at the red tail of the emission spectrum of the fluorophore to deplete the excited state by stimulated emission. Since the stimulated emission cross-section follows practically the same dependency with wavelength as the emission, this configuration involves very [...]